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I am interested in the ab105135 kit. The protocol states that formation of Glutamate is measured by by a colorless probe emitting light at 450 nm. Can you tell me the nature of this probe (chemical structure and mechanism) How exactly does this kit work?
Asked on Dec 14 2012
The identity of all the kit components is proprietary information. The mechanism of the reaction is exactly as mentioned on the datasheet. The substrate is a sequence the AST and the other enzymes in the provided mix will act upon. The probe will interact with the glutamate in the presence of the enzymes and produce a coloured product which can be measured.
Answered on Dec 14 2012