Overview

  • Product name

    Aspartate Aminotransferase Activity Assay Kit (Fluorometric)
    See all Aspartate Aminotransferase kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Other biological fluids
  • Assay type

    Enzyme activity
  • Sensitivity

    2 mU/ml
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Aspartate Aminotransferase Activity Assay Kit / AST Assay Kit (Fluorometric) ab138878 provides a quick and sensitive assay for AST activity in biological samples.


    In the AST assay protocol, AST catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:


    Aspartate (Asp) + α-ketoglutarate ? oxaloacetate + glutamate (Glu)


    The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle.


    The assay signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). The AST activity assay can detect as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.

  • Notes

    Aspartate aminotransferase (AST), also called Glutamic Oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism.

  • Platform

    Microplate reader

Properties

Images

  • AST dose response was measured with ab138878 in a 96-well black plate using a fluorescence microplate reader. As low as 2 mU/ml AST can be detected with 60 min incubation (n=3) at room temperature.

Protocols

References

ab138878 has not yet been referenced specifically in any publications.

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