Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Other biological fluids, Serum
- Sensitivity: 2 mU/ml
Product nameAspartate Aminotransferase Activity Assay Kit (Fluorometric)
See all Aspartate Aminotransferase kits
Sample typeSerum, Other biological fluids
Assay typeEnzyme activity
Species reactivityReacts with: Mammals, Other species
Aspartate Aminotransferase Activity Assay Kit / AST Assay Kit (Fluorometric) ab138878 provides a quick and sensitive assay for AST activity in biological samples.
In the AST assay protocol, AST catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate:
Aspartate (Asp) + α-ketoglutarate ? oxaloacetate + glutamate (Glu)
The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle.
The assay signal can be read by a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (optimal Ex/Em = 540 nm/590 nm). The AST activity assay can detect as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.
Aspartate aminotransferase (AST), also called Glutamic Oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an α-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit AST Assay Buffer 1 x 10ml AST Assay Enzyme Mixture 1 vial AST Positive Control 1 vial NAD 1 vial
FunctionPlays a key role in amino acid metabolism.
Sequence similaritiesBelongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
- Information by UniProt
- Aspartate aminotransferase
- Aspartate aminotransferase 1
ab138878 has been referenced in 2 publications.
- Humpton TJ et al. Differential requirements for MDM2 E3 activity during embryogenesis and in adult mice. Genes Dev 35:117-132 (2021). PubMed: 33334825
- Hwang I et al. Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis. Cell Biosci 11:3 (2021). PubMed: 33407858