Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
Key features and details
- Mouse monoclonal [C1] to Aspartate Aminotransferase
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG2a
Overview
-
Product name
Anti-Aspartate Aminotransferase antibody [C1]
See all Aspartate Aminotransferase primary antibodies -
Description
Mouse monoclonal [C1] to Aspartate Aminotransferase -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Horse, Cow, Chimpanzee, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human Aspartate Aminotransferase aa 1-450. N-terminal His Tag. Expressed in E.coli.
Database link: P17174 -
Positive control
- WB: Recombinant Aspartate Aminotransferase protein; Human and mouse liver lysates; Rat liver, brain and heart lysate; Pig brain and heart lysate. HAP1, HeLa and Raw264.7 cell lysates. IHC-P: Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 30 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 5 µg/ml.
|
Notes |
---|
IHC-P
Use a concentration of 5 - 30 µg/ml. |
WB
Use a concentration of 0.5 - 5 µg/ml. |
Target
-
Function
Plays a key role in amino acid metabolism. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 450664 Chimpanzee
- Entrez Gene: 281206 Cow
- Entrez Gene: 101865666 Cynomolgus monkey
- Entrez Gene: 100060793 Horse
- Entrez Gene: 2805 Human
- Entrez Gene: 14718 Mouse
- Entrez Gene: 100173864 Orangutan
- Entrez Gene: 396967 Pig
see all -
Alternative names
- AATC_HUMAN antibody
- Aspartate aminotransferase 1 antibody
- Aspartate aminotransferase antibody
see all
Images
-
All lanes : Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 0.5 µg/ml
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : GOT1 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.Lanes 1 - 4: Merged signal (red and green). Green - ab239487 observed at 46 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab239487 was shown to react with Aspartate Aminotransferase in wild-type HAP1 cells in western blot. Loss of signal was observed when GOT1 knockout sample was used. Wild-type and GOT1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab239487 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 2 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Pig heart lysate
Secondary
All lanes : HRP-linked Rabbit anti-Mouse IgG at 1/5000 dilution -
Paraffin-embedded human kidney tissue stained for Aspartate Aminotransferase using ab239487 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat heart lysate
Secondary
HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml -
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat brain lysate
Secondary
HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml -
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat liver lysate
Secondary
HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml -
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Mouse liver lysate
Secondary
HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml -
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Human liver lysate
Secondary
HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml -
Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 5 µg/ml + Recombinant human aspartate aminotransferase protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab239487 has not yet been referenced specifically in any publications.