Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Product nameAspartate Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Other biological fluids, Tissue Extracts
Sensitivity> 2 µM
Range2 µM - 200 µM
Assay time0h 40m
Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Aspartate Assay Buffer WM 1 x 25ml Aspartate Enzyme Mix Green 1 vial Aspartate Probe (in DMSO solution) Red 1 x 0.2ml Aspartate Standard Yellow 1 x 0.1ml Conversion Mix Purple 1 vial Serum CleanUp Mix Blue 1 vial
Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).
Fluorometric Standard Curve Performed as Described in the Protocol
Colorimetric Standard Curve Performed as Described in the Protocol
ab102512 has been referenced in 9 publications.
- Wang J et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). PubMed: 30591220
- Hong R et al. Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation. Mol Oncol 13:959-977 (2019). PubMed: 30714292
- Agudelo LZ et al. Skeletal muscle PGC-1a1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance. Nat Commun 10:2767 (2019). PubMed: 31235694
- Sun W et al. Aspulvinone O, a natural inhibitor of GOT1 suppresses pancreatic ductal adenocarcinoma cells growth by interfering glutamine metabolism. Cell Commun Signal 17:111 (2019). PubMed: 31470862
- Cremer J et al. Chemotaxis as a navigation strategy to boost range expansion. Nature 575:658-663 (2019). PubMed: 31695195
- Tumurkhuu G et al. Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1ß Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis. Cell Metab 28:432-448.e4 (2018). PubMed: 29937375
- Fu X et al. Spatial self-organization resolves conflicts between individuality and collective migration. Nat Commun 9:2177 (2018). PubMed: 29872053
- Zhou J et al. Inhibition of LIN28B impairs leukemia cell growth and metabolism in acute myeloid leukemia. J Hematol Oncol 10:138 (2017). PubMed: 28693523
- Cao LL et al. Control of mitochondrial function and cell growth by the atypical cadherin Fat1. Nature 539:575-578 (2016). PubMed: 27828948