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Abcam's Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
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L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.
|Aspartate Assay Buffer||WM||1 x 25ml|
|Aspartate Enzyme Mix||Green||1 vial|
|Aspartate Probe (in DMSO solution)||Red||1 x 0.2ml|
|Aspartate Standard||Yellow||1 x 0.1ml|
|Conversion Mix||Purple||1 vial|
|Serum CleanUp Mix||Blue||1 vial|
Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).
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