Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Product nameAspartate Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Other biological fluids, Tissue Extracts
Sensitivity> 2 µM
Range2 µM - 200 µM
Assay time0h 40m
Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM).
This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.
L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Aspartate Assay Buffer WM 1 x 25ml Aspartate Enzyme Mix Green 1 vial Aspartate Probe (in DMSO solution) Red 1 x 0.2ml Aspartate Standard Yellow 1 x 0.1ml Conversion Mix Purple 1 vial Serum CleanUp Mix Blue 1 vial
Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).
Fluorometric Standard Curve Performed as Described in the Protocol
Colorimetric Standard Curve Performed as Described in the Protocol
ab102512 has been referenced in 9 publications.
- Wang J et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). PubMed: 30591220
- Hong R et al. Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation. Mol Oncol 13:959-977 (2019). PubMed: 30714292
- Agudelo LZ et al. Skeletal muscle PGC-1a1 reroutes kynurenine metabolism to increase energy efficiency and fatigue-resistance. Nat Commun 10:2767 (2019). PubMed: 31235694
- Sun W et al. Aspulvinone O, a natural inhibitor of GOT1 suppresses pancreatic ductal adenocarcinoma cells growth by interfering glutamine metabolism. Cell Commun Signal 17:111 (2019). PubMed: 31470862
- Cremer J et al. Chemotaxis as a navigation strategy to boost range expansion. Nature 575:658-663 (2019). PubMed: 31695195
- Tumurkhuu G et al. Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1ß Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis. Cell Metab 28:432-448.e4 (2018). PubMed: 29937375
- Fu X et al. Spatial self-organization resolves conflicts between individuality and collective migration. Nat Commun 9:2177 (2018). PubMed: 29872053
- Zhou J et al. Inhibition of LIN28B impairs leukemia cell growth and metabolism in acute myeloid leukemia. J Hematol Oncol 10:138 (2017). PubMed: 28693523
- Cao LL et al. Control of mitochondrial function and cell growth by the atypical cadherin Fat1. Nature 539:575-578 (2016). PubMed: 27828948