Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
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Overview
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Product nameAnti-Aspartate beta hydroxylase antibody [EPR10351]
See all Aspartate beta hydroxylase primary antibodies -
DescriptionRabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797 -
Positive control
- HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10351
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab172475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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RelevanceASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins.
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Cellular localizationEndoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein.
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Database links
- Entrez Gene: 444 Human
- Omim: 600582 Human
- SwissProt: Q12797 Human
- Unigene: 332422 Human
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Alternative names
- A beta H J J antibody
- AAH antibody
- ASP beta hydroxylase antibody
see all
Images
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![Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194726-ab1724751.jpg)
Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194728-ab1724753.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194727-ab1724752.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194729-ab1724754.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194730-ab1724755.JPG)
Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
ab172475 has not yet been referenced specifically in any publications.
