Recombinant
RabMAb

Recombinant Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

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Overview

  • Product name

    Anti-Aspartate beta hydroxylase antibody [EPR10351]
    See all Aspartate beta hydroxylase primary antibodies

  • Description

    Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q12797

  • Positive control

    • HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins.

    • Cellular localization

      Endoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein.

    • Database links

      • Alternative names

        • A beta H J J antibody
        • AAH antibody
        • ASP beta hydroxylase antibody
        • Aspartyl/asparaginyl beta hydroxylase antibody
        • ASPH antibody
        • ASPH_HUMAN antibody
        • BAH antibody
        • Cardiac junctin antibody
        • CASQ2BP1 antibody
        • HAAH antibody
        • Humbug antibody
        • JCTN antibody
        • junctin antibody
        • Peptide aspartate beta dioxygenase antibody
        see all

      Images

      • Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : A549 cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 86 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

        Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

        Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

        Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red).  DAPI nuclear staining (blue).

      • Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
        Flow Cytometry - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

        Flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).

      References

      ab172475 has not yet been referenced specifically in any publications.

      Publishing research using ab172475? Please let us know so that we can cite the reference in this datasheet.

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