Recombinant Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
![Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194726-anti-aspartate-beta-hydroxylase-antibody-epr10351-western-blot.jpg "Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aspartate beta hydroxylase antibody [EPR10351]
See all Aspartate beta hydroxylase primary antibodies -
Description
Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797 -
Positive control
- HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10351 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
Target
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Relevance
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins. -
Cellular localization
Endoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein. -
Database links
- Entrez Gene: 444 Human
- Omim: 600582 Human
- SwissProt: Q12797 Human
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Alternative names
- A beta H J J antibody
- AAH antibody
- ASP beta hydroxylase antibody
see all
Images
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Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194728-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194727-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194729-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-194730-anti-aspartate-beta-hydroxylase-antibody-epr10351-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylasewith ab172475at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
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![Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.com/ps/products/172/ab172475/Images/ab172475-6-benefits-of-recombinant-antibodies.png)
Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab172475 has been referenced in 1 publication.
- Chen X et al. miR-135a acts as a tumor suppressor by targeting ASPH in endometrial cancer. Int J Clin Exp Pathol 12:3384-3389 (2019). PubMed: 31934181
