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  1. Link

    aspartate-beta-hydroxylase-antibody-epr10351-ab172475.pdf

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Neuroscience Neurotransmission Calcium Signaling Calcium Channels Ryanodine Receptors
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RecombinantRabMAb

Recombinant Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

  • Datasheet
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Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
  • Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
  • Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Aspartate beta hydroxylase antibody [EPR10351]
    See all Aspartate beta hydroxylase primary antibodies
  • Description

    Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q12797

  • Positive control

    • HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10351
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Channels
    • Ryanodine Receptors
    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • IP3R
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other

Associated products

  • Alternative Versions

    • Anti-Aspartate beta hydroxylase antibody [EPR10351] - BSA and Azide free (ab249694)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IP.

Target

  • Relevance

    ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins.
  • Cellular localization

    Endoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein.
  • Database links

    • Entrez Gene: 444 Human
    • Omim: 600582 Human
    • SwissProt: Q12797 Human
    • Alternative names

      • A beta H J J antibody
      • AAH antibody
      • ASP beta hydroxylase antibody
      • Aspartyl/asparaginyl beta hydroxylase antibody
      • ASPH antibody
      • ASPH_HUMAN antibody
      • BAH antibody
      • Cardiac junctin antibody
      • CASQ2BP1 antibody
      • HAAH antibody
      • Humbug antibody
      • JCTN antibody
      • junctin antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Images

    • Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 86 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

      Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

      Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red).  DAPI nuclear staining (blue).

    • Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

      Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylasewith ab172475at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green). 

       

       

    • Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
      Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab172475? Please let us know so that we can cite the reference in this datasheet.

    ab172475 has been referenced in 1 publication.

    • Chen X  et al. miR-135a acts as a tumor suppressor by targeting ASPH in endometrial cancer. Int J Clin Exp Pathol 12:3384-3389 (2019). PubMed: 31934181

    Customer reviews and Q&As

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