Recombinant Anti-Aspartyl Aminopeptidase antibody [EPR10301] (ab154805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10301] to Aspartyl Aminopeptidase
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aspartyl Aminopeptidase antibody [EPR10301]
See all Aspartyl Aminopeptidase primary antibodies -
Description
Rabbit monoclonal [EPR10301] to Aspartyl Aminopeptidase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Aspartyl Aminopeptidase. The exact sequence is proprietary.
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Positive control
- MCF7, 293T, K562 and A549 cell lysates; 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10301 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Function
Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M18 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23549 Human
- Entrez Gene: 13437 Mouse
- Entrez Gene: 301529 Rat
- Omim: 611367 Human
- SwissProt: Q9ULA0 Human
- SwissProt: Q9Z2W0 Mouse
- Unigene: 258551 Human
- Unigene: 24680 Mouse
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Alternative names
- Aspartyl aminopeptidase antibody
- ASPEP antibody
- DAP antibody
see all
Images
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All lanes : Anti-Aspartyl Aminopeptidase antibody [EPR10301] (ab154805) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : 293T lysate
Lane 3 : K562 lysate
Lane 4 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cells staining Aspartyl Aminopeptidase using ab154805 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154805 has been referenced in 2 publications.
- Koumangoye R & Delpire E DNPEP is not the only peptidase that produces SPAK fragments in kidney. Physiol Rep 5:N/A (2017). PubMed: 29122955
- Markadieu N et al. Short forms of Ste20-related proline/alanine-rich kinase (SPAK) in the kidney are created by aspartyl aminopeptidase (Dnpep)-mediated proteolytic cleavage. J Biol Chem 289:29273-84 (2014). PubMed: 25164821