Overview

  • Product name
  • Description
    Rabbit polyclonal to Aspergillus
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-P, Conjugationmore details
  • Species reactivity
    Reacts with: Aspergillus nidulans
  • Immunogen

    Tissue/ cell preparation. Soluble extract from A. fumigatus, A. flavus, A. niger and A. terreus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >95% pure
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Conjugation Use at an assay dependent concentration.

Target

  • Relevance
    The genus Aspergillus includes over 185 species. Around 20 species have been reported as causative agents of opportunistic infections in humans. Among these, Aspergillus fumigatus is the most commonly isolated species, followed by Aspergillus flavus. Aspergillus fumigatus is the major cause of aspergillosis. This organism causes both invasive and allergic aspergillosis. Aspergillus also produce fungal toxins called mycotoxins. Aflatoxin is produced by Aspergillus flavus as it grows on corn and peanuts. The toxin is poisonous to humans by ingestion and causes liver disease. Aspergillus nidulans can produce the mycotoxin sterigmatocystin. This toxin has been shown to produce liver and kidney damage in lab animals. Aspergillus oschraceus,found in grains, soil and salted food products can produce a kidney toxin called oschratoxin A, which may produce oschratoxicosis in humans. Ochratoxin may also be produced by other aspergillus and penicillium species. Other toxins that can be produced by this fungus include penicillic acid, xanthomegnin and viomellein. Aspergillus infections have a very high mortality rate. Their incidence is growing because of the increased number of immunocompromised patients. Previous to antibodies such as these, special stains were used to identify aspergillus. Aspergillus oryzae and Aspergillus niger are used extensively in industrial scale fermentation to produce enzymes for processing household food and drink products.

Images

  • ab20419, staining Aspergillus (green) in lung tissue from a murine model of corticosteroid-induced invasive pulmonary aspergillosis, by Immunohistochemistry.

    Tissue was fixed in cold acetone for 15 min, followed by washing and blocking with normal serum block at RT for 30 min. Sections were incubated overnight at 4°C with primary antibody. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/00) was used as the secondary antibody.
  • ab20419, staining Aspergillus (green) in lung tissue from a murine model of chemotherapy-induced invasive pulmonary aspergillosis, by Immunohistochemistry.

    Tissue was fixed in cold acetone for 15 min, followed by washing and blocking with normal serum block at RT for 30 min. Sections were incubated overnight at 4°C with primary antibody. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/00) was used as the secondary antibody.

References

This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us. Sorry for the delay.

It seems that one of your colleague is already in contact with us. We have provided all the information we have regarding this product. I am sorry we do not have any additional information.

The possible reason of no saturation could be because many fungal antigens are highly cross linked polysaccharides and as such do not coat well in ELISA. The remedy for this might be drying the antigen suspensions down directly in the wells of the ELISA plate. This binds them strongly in the wells. Before use, the wells should be washed well with a detergent buffer to remove material that is not strongly bound.”

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Gracias por tu paciencia.

Me confirman mis compañeros del laboratorio que el extracto soluble usado como inmunógeno de este anticuerpo es una mezcla de esporas e hifa procedentes de A. fumigatus, A. flavus, A. niger and A. terreus.

Espero que esta información sea de utilidad. No dudes en contactarnos si tienes cualquier otra consulta.

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Answer

Gracias por contactar con Abcam.

Mi compañera no se encuentra hoy en la oficina.

Thank you for your inquiry.

The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. For polyclonal antibodies epitope mapping is not performed (only for monoclonals).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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Answer

I am sorry for the delay in getting back to you with the ELISA protocol information for the Anti-Aspergillus antibody (ab20419). I now have further information to share with you. The antibody has been used as follows:

1. Coat plate with a commercial Aspergillus mycelial extractovernight in PBS at room temperature

2. Wash wells 3x with PBS + 0.3% tween-20 (PBST)

3. Add serial two-fold dilutions of theantibodyab20419 startingat 1:100 in PBST & incubate for 30 minutesat room temperature

4. Wash wells 3x with PBS + 0.3% tween-20 (PBST)

5. Add secondary antibody-HRP conjugate diluted in PBST and incubate for 30 minutesat room temperature

6. Wash wells 3x with PBS + 0.3% tween-20 (PBST)

7. Add substrate (TMB) for 1-2 minutes, then stop with H2S04 addition.

8. Read wells at 450 nm.

This information is intended as a starting point only. You will need to perform further optimisation to obtain the best results using your own system, for example, a blocking step would be beneficial to reduce the background and the concentration of primary and secondary antibody required.

I hope this information has been of help. If you require any further information , please do not hesitate to contact us again.

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Answer

Thank you for your enquiry. Unfortunately, we have no antibodies I can recommend that will specifically detect intact aspergillus spores. However, I can offer the following information regarding the antibodies you have suggested: In immunofluorescence, ab34953 stains the cell wall very well,and the spores themselves less well (a weaker signal is seen). The other antibody,ab20419, has been tested with (and will recognize) Aspergillus niger spores, but we do not know if the epitopes recognized are located on the spore surface (cell wall). Therefore we cannot guarantee that this antibody will recognize intact spores. I am sorry I am not able to recommend a specific product on this occasion, but I hope this information is helpful. Please do not hesitate to contact us again should the customer have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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