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  1. Link

    aspergillus-antibody-ab34953.pdf

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Microbiology Organism Eukaryotic Microorganism Fungi Aspergillus
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Anti-Aspergillus antibody (ab34953)

  • Datasheet
  • SDS
Submit a review Q&A (1)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Rabbit polyclonal to Aspergillus
  • Suitable for: ELISA
  • Isotype: IgG

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Overview

  • Product name

    Anti-Aspergillus antibody
    See all Aspergillus primary antibodies
  • Description

    Rabbit polyclonal to Aspergillus
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with soluble proteins from common Aspergillus species.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reactive with soluble proteins from common
  • Immunogen

    Tissue, cells or virus corresponding to Aspergillus. Soluble extract from A. fumigates, A. flavus, A. niger and A. terreus.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    This antibody is greater than 95% pure.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Fungi
    • Aspergillus

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab34953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Notes
Application notes
Dot blot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Relevance

    The genus Aspergillus includes over 185 species. Around 20 species have been reported as causative agents of opportunistic infections in humans. Among these, Aspergillus fumigatus is the most commonly isolated species, followed by Aspergillus flavus. Aspergillus fumigatus is the major cause of aspergillosis. This organism causes both invasive and allergic aspergillosis. Aspergillus also produce fungal toxins called mycotoxins. Aflatoxin is produced by Aspergillus flavus as it grows on corn and peanuts. The toxin is poisonous to humans by ingestion and causes liver disease. Aspergillus nidulans can produce the mycotoxin sterigmatocystin. This toxin has been shown to produce liver and kidney damage in lab animals. Aspergillus oschraceus,found in grains, soil and salted food products can produce a kidney toxin called oschratoxin A, which may produce oschratoxicosis in humans. Ochratoxin may also be produced by other aspergillus and penicillium species. Other toxins that can be produced by this fungus include penicillic acid, xanthomegnin and viomellein. Aspergillus infections have a very high mortality rate. Their incidence is growing because of the increased number of immunocompromised patients. Previous to antibodies such as these, special stains were used to identify aspergillus. Aspergillus oryzae and Aspergillus niger are used extensively in industrial scale fermentation to produce enzymes for processing household food and drink products.

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab34953? Please let us know so that we can cite the reference in this datasheet.

ab34953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

A customer is looking for an antibody that will recognize aspergillus cell wall. Their assay involves labeling fangas sphores with a fluorophore and then capturing with antibodies conjugated to microspheres (they have the conjugation procedure running). The important factor is that the ab will recognize intact sphores, i.e. the cell wall. In ab20419 and ab34953 the immunogen is a soluble extract from several aspergillus types. Do you have any indication regarding the ability of this ab to recognize intact aspergillus sphores? Thank you,

Read More

Abcam community

Verified customer

Asked on Dec 17 2006

Answer

Thank you for your enquiry. Unfortunately, we have no antibodies I can recommend that will specifically detect intact aspergillus spores. However, I can offer the following information regarding the antibodies you have suggested: In immunofluorescence, ab34953 stains the cell wall very well,and the spores themselves less well (a weaker signal is seen). The other antibody,ab20419, has been tested with (and will recognize) Aspergillus niger spores, but we do not know if the epitopes recognized are located on the spore surface (cell wall). Therefore we cannot guarantee that this antibody will recognize intact spores. I am sorry I am not able to recommend a specific product on this occasion, but I hope this information is helpful. Please do not hesitate to contact us again should the customer have any further questions.

Read More

Abcam Scientific Support

Answered on Dec 20 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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