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A customer is looking for an antibody that will recognize aspergillus cell wall. Their assay involves labeling fangas sphores with a fluorophore and then capturing with antibodies conjugated to microspheres (they have the conjugation procedure running). The important factor is that the ab will recognize intact sphores, i.e. the cell wall. In ab20419 and ab34953 the immunogen is a soluble extract from several aspergillus types. Do you have any indication regarding the ability of this ab to recognize intact aspergillus sphores? Thank you,
Asked on Dec 17 2006
Thank you for your enquiry. Unfortunately, we have no antibodies I can recommend that will specifically detect intact aspergillus spores. However, I can offer the following information regarding the antibodies you have suggested: In immunofluorescence, ab34953 stains the cell wall very well,and the spores themselves less well (a weaker signal is seen). The other antibody,ab20419, has been tested with (and will recognize) Aspergillus niger spores, but we do not know if the epitopes recognized are located on the spore surface (cell wall). Therefore we cannot guarantee that this antibody will recognize intact spores. I am sorry I am not able to recommend a specific product on this occasion, but I hope this information is helpful. Please do not hesitate to contact us again should the customer have any further questions.
Answered on Dec 20 2006