Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Product nameAspergillus fumigatus IgM ELISA kit
Intra-assay Sample n Mean SD CV% sample 24 0.436nM 2.65% sample 24 0.645nM 3.63% sample 24 1.203nM 1.83% Inter-assay Sample n Mean SD CV% sample 12 15.41nM 5.76% sample 12 31.75nM 6.61% sample 12 6.61nM 6.19%
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Aspergillus fumigatus IgM ELISA kit (ab247191) is designed for the qualitative determination of IgM class antibodies against Aspergillus fumigatus in human serum or plasma (citrate, heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Aspergillus fumigatus is a fungus of the genus Aspergillus and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency. A. fumigatus, a saprotroph, is widespread in nature and is typically found in soil and decaying organic matter. Colonies of the fungus produce thousands of small conidia (2-3 μm) that readily become airborne. The fungus is capable of growth at temperatures up to 50 °C, with conidia surviving at 70 °C. Humans are continuously in contact with these asexual spores and it is estimated that an individual inhales several hundred conidia each day; typically, these are quickly eliminated by the immune system. Aspergillosis develops mainly in individuals who are immunocompromised, either from disease or from immunosuppressive drugs, and is a leading cause of death in acute leukemia and hematopoietic stem cell transplantation.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml anti-human IgM HRP conjugate 1 x 20ml Aspergillus fumigatus Coated Microplate (IgM) 1 unit Cover Foil 1 unit IgM Cut-off Control 1 x 3ml IgM Negative Control 1 x 2ml IgM Positive Control 1 x 2ml IgM Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab247191 has not yet been referenced specifically in any publications.