Anti-ASPP1 antibody (ab51831)

All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilution

Lane 1 : Non-transfected control
Lane 2 : MCF7 cells over expressing ASPP1
Lane 3 : MCF7 cells overexpressing ASPP2

Predicted band size: 121 kDa
Observed band size: 120,50 kDa (why is the actual band size different from the predicted?)
Additional bands at: 121 kDa. We are unsure as to the identity of these extra bands.



Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1/1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.

ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.