The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/500 - 1/3000. Detects a band of approximately 120 kDa (predicted molecular weight: 121 kDa).
1/2500 - 1/10000.
Use at an assay dependent concentration.
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Reduced expression in breast carcinomas expressing a wild-type TP53 protein.
Belongs to the ASPP family. Contains 2 ANK repeats. Contains 1 SH3 domain.
The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
Cell extracts were electrophoresed and transferred to
nitrocellulose. The membrane was probed with the primary
antibody at a 1/1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary
experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.