Anti-ASPP1 antibody (ab51831)
Key features and details
- Rabbit polyclonal to ASPP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASPP1 antibody
See all ASPP1 primary antibodies -
Description
Rabbit polyclonal to ASPP1 -
Host species
Rabbit -
Specificity
Minimal reactivity occurs against ASPP2. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to an internal sequence of ASPP1 (Human)
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Positive control
- MCF7 cells extracts
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab51831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | 1/500 - 1/3000. Detects a band of approximately 120 kDa (predicted molecular weight: 121 kDa). |
Target
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Function
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. -
Tissue specificity
Reduced expression in breast carcinomas expressing a wild-type TP53 protein. -
Sequence similarities
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain. -
Domain
The ankyrin repeats and the SH3 domain are required for specific interactions with TP53. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear. - Information by UniProt
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Database links
- Entrez Gene: 23368 Human
- Entrez Gene: 21981 Mouse
- Omim: 606455 Human
- SwissProt: Q96KQ4 Human
- SwissProt: Q62415 Mouse
- Unigene: 709297 Human
- Unigene: 313076 Mouse
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Alternative names
- Apoptosis-stimulating of p53 protein 1 antibody
- ASPP1_HUMAN antibody
- p53BP2 like antibody
see all
Images
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Western blot - Anti-ASPP1 antibody (ab51831)All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilution
Lane 1 : Non-transfected control
Lane 2 : MCF7 cells over expressing ASPP1
Lane 3 : MCF7 cells overexpressing ASPP2
Predicted band size: 121 kDa
Observed band size: 120,50 kDa why is the actual band size different from the predicted?
Additional bands at: 121 kDa. We are unsure as to the identity of these extra bands.
Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1/1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. -
Immunocytochemistry/ Immunofluorescence - Anti-ASPP1 antibody (ab51831)ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab51831 has not yet been referenced specifically in any publications.
