Product nameAnti-ASPP1 antibody
See all ASPP1 primary antibodies
DescriptionRabbit polyclonal to ASPP1
SpecificityMinimal reactivity occurs against ASPP2.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to an internal sequence of ASPP1 (Human)
- MCF7 cells extracts
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Sterile filtered.
Our Abpromise guarantee covers the use of ab51831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/3000. Detects a band of approximately 120 kDa (predicted molecular weight: 121 kDa).|
|ELISA||1/2500 - 1/10000.|
|IHC-P||Use at an assay dependent concentration.|
FunctionRegulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Tissue specificityReduced expression in breast carcinomas expressing a wild-type TP53 protein.
Sequence similaritiesBelongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
- Information by UniProt
- Apoptosis-stimulating of p53 protein 1 antibody
- ASPP1_HUMAN antibody
- p53BP2 like antibody
All lanes : Anti-ASPP1 antibody (ab51831) at 1/1000 dilution
Lane 1 : Non-transfected control
Lane 2 : MCF7 cells over expressing ASPP1
Lane 3 : MCF7 cells overexpressing ASPP2
Predicted band size: 121 kDa
Observed band size: 120,50 kDa why is the actual band size different from the predicted?
Additional bands at: 121 kDa. We are unsure as to the identity of these extra bands.
Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1/1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.
ICC/IF image of ab51831 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab51831 has not yet been referenced specifically in any publications.