Key features and details
- Rabbit polyclonal to ASPP1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASPP1 antibody
See all ASPP1 primary antibodies
DescriptionRabbit polyclonal to ASPP1
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A synthetic peptide corresponding to a region between residue 50 and 100 of human ASPP1 (NP_056131.1)
- Whole cell lysate from 293T cells and Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Tissue specificityReduced expression in breast carcinomas expressing a wild-type TP53 protein.
Sequence similaritiesBelongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
- Information by UniProt
- Apoptosis-stimulating of p53 protein 1 antibody
- ASPP1_HUMAN antibody
- p53BP2 like antibody
All lanes : Anti-ASPP1 antibody (ab71163) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 5 µg
Lane 4 : Whole cell lysate from Hela cells at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunoprecipitation/ Western Blot of ASPP1.
Lane 1: ab71163 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 10 seconds.
ICC/IF image of ab71163 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71163, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab71163 has been referenced in 2 publications.
- Chen D et al. E2f2 induces cone photoreceptor apoptosis independent of E2f1 and E2f3. Cell Death Differ 20:931-940 (2013). PubMed: 23558950
- Vigneron AM & Vousden KH An indirect role for ASPP1 in limiting p53-dependent p21 expression and cellular senescence. EMBO J : (2011). WB ; Human . PubMed: 22068052