Product nameAnti-ASPP1 antibody
See all ASPP1 primary antibodies
DescriptionRabbit polyclonal to ASPP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
A synthetic peptide corresponding to a region between residue 475 and 525 of human ASPP1 (NP_056131.1)
- Whole cell lysate from 293T cells and Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 121 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.
Tissue specificityReduced expression in breast carcinomas expressing a wild-type TP53 protein.
Sequence similaritiesBelongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for specific interactions with TP53.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic. Some fraction is nuclear.
- Information by UniProt
- Apoptosis-stimulating of p53 protein 1 antibody
- ASPP1_HUMAN antibody
- p53BP2 like antibody
All lanes : Anti-ASPP1 antibody (ab71164) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 5 µg
Lane 4 : Whole cell lysate from Hela cells at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunoprecipitation/ Western Blot of ASPP1.
Lane 1: ab71164 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
All lanes: Whole cell lysate from 293T cells at 1mg for IP, 20% of IP loaded. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab71164 has not yet been referenced specifically in any publications.