Validated using a knockout cell line

Anti-ASS1 antibody [2B10] (ab124465)

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Overview

  • Product name

    Anti-ASS1 antibody [2B10]
    See all ASS1 primary antibodies

  • Description

    Mouse monoclonal [2B10] to ASS1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey

  • Immunogen

    Purified recombinant fragment of Human ASS1, expressed in E. Coli.

  • Positive control

    • HeLa and Jurkat cells; Human ASS1 recombinant protein; A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab124465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.

  • Involvement in disease

    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.

  • Sequence similarities

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Images

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).
  • ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA using ab124465.
  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : L1210 cell lysate
    Lane 4 : MOLT4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : A549 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)

    Predicted band size: 47 kDa

References

This product has been referenced in:

  • Silberman A  et al. Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer. Cancer Res 79:518-533 (2019). Read more (PubMed: 30573518) »
  • Li T  et al. Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Upregulates Argininosuccinate Synthase 1, a Rate-Limiting Enzyme of the Citrulline-Nitric Oxide Cycle, To Activate the STAT3 Pathway and Promote Growth Transformation. J Virol 93:N/A (2019). Read more (PubMed: 30463977) »
See all 3 Publications for this product

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