Key features and details
- Mouse monoclonal [2B10] to ASS1
- Suitable for: WB, ELISA, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG1
Product nameAnti-ASS1 antibody [2B10]
See all ASS1 primary antibodies
DescriptionMouse monoclonal [2B10] to ASS1
Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Purified recombinant fragment of Human ASS1, expressed in E. Coli.
- ICC: HeLa cells. Flow Cyt: Jurkat cells. WB: Human ASS1 recombinant protein; HAP1, A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.
This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
KO cell lines
Our Abpromise guarantee covers the use of ab124465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200 - 1/1000.|
PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
Involvement in diseaseDefects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
Sequence similaritiesBelongs to the argininosuccinate synthase family. Type 1 subfamily.
- Information by UniProt
- Argininosuccinate synthase 1 antibody
- Argininosuccinate synthase antibody
- Argininosuccinate synthetase 1 antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.
ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).
ELISA using ab124465.
All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution
Lane 1 : A431 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : L1210 cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : A549 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : COS7 cell lysate
Predicted band size: 47 kDa
Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)
Predicted band size: 47 kDa
ab124465 has been referenced in 4 publications.
- Silberman A et al. Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer. Cancer Res 79:518-533 (2019). PubMed: 30573518
- Li T et al. Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Upregulates Argininosuccinate Synthase 1, a Rate-Limiting Enzyme of the Citrulline-Nitric Oxide Cycle, To Activate the STAT3 Pathway and Promote Growth Transformation. J Virol 93:N/A (2019). PubMed: 30463977
- Ochocki JD et al. Arginase 2 Suppresses Renal Carcinoma Progression via Biosynthetic Cofactor Pyridoxal Phosphate Depletion and Increased Polyamine Toxicity. Cell Metab 27:1263-1280.e6 (2018). PubMed: 29754953
- Ohshima K et al. Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer. Sci Rep 7:45504 (2017). Human . PubMed: 28358054