Key features and details
- Goat polyclonal to ASS1
- Suitable for: WB, IP, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASS1 antibody
See all ASS1 primary antibodies
DescriptionGoat polyclonal to ASS1
Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
- Recombinant Human ASS1 protein (ab116472) can be used as a positive control in WB. Human kidney and liver tissue; Human kidney lysate
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 47 kDa.|
|IP||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
Involvement in diseaseDefects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
Sequence similaritiesBelongs to the argininosuccinate synthase family. Type 1 subfamily.
- Information by UniProt
- Argininosuccinate synthase 1 antibody
- Argininosuccinate synthase antibody
- Argininosuccinate synthetase 1 antibody
ab109753, at 2.5 µg/ml, staining ASS1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.
ab109753, at 2.5 µg/ml, staining ASS1 in formalin fixed, paraffin embedded Human liver tissue by Immunohistochemistry.
Anti-ASS1 antibody (ab109753) at 0.03 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
Predicted band size: 47 kDa
Primary incubation was 1 hour. Detected by chemiluminescence
ab109753 has been referenced in 1 publication.
- Marini JC et al. The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig. Am J Physiol Endocrinol Metab 313:E233-E242 (2017). PubMed: 28611027