• Product name
  • Description
    Rabbit polyclonal to ASS1
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ASS1 aa 199-229 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P00966

  • Positive control
    • Mouse kidney tissue lysates, Hela cell line lysates, Human colon carcinoma tissue, widr cells and Hela cells.



Our Abpromise guarantee covers the use of ab175607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 47 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Pathway
    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
  • Involvement in disease
    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
  • Sequence similarities
    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all


  • Anti-ASS1 antibody (ab175607) at 1/1000 dilution + Hela cell line lysates at 35 µg

    Predicted band size: 47 kDa

  •  Immunofluorescence analysis of HeLa cells labeling ASS1 with ab175607 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-ASS1 antibody (ab175607) at 1/1000 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size: 47 kDa

  •  Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human colon carcinoma labeling ASS1with ab175607 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  •  Flow Cytometry analysis of Widr cells labeling ASS1 with ab175607 at 1/10 (bottom histogram) and a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


This product has been referenced in:
  • Lange SM  et al. l-Citrulline Metabolism in Mice Augments CD4+T Cell Proliferation and Cytokine ProductionIn Vitro, and Accumulation in the Mycobacteria-Infected Lung. Front Immunol 8:1561 (2017). Read more (PubMed: 29201027) »
See 1 Publication for this product

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