Key features and details
- Rabbit polyclonal to ASS1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ASS1 antibody
See all ASS1 primary antibodies
DescriptionRabbit polyclonal to ASS1
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
- Human kidney tissue; HeLa and WiDr cells; Mouse kidney and HeLa lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab191165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 47 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
Involvement in diseaseDefects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
Sequence similaritiesBelongs to the argininosuccinate synthase family. Type 1 subfamily.
- Information by UniProt
- Argininosuccinate synthase 1 antibody
- Argininosuccinate synthase antibody
- Argininosuccinate synthetase 1 antibody
Anti-ASS1 antibody (ab191165) at 1/1000 dilution + HeLa cell lysate at 35 µg
Predicted band size: 47 kDa
Anti-ASS1 antibody (ab191165) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg
Predicted band size: 47 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ASS1 with ab191165 at 1/75 dilution.
Flow cytometric analysis of WiDr cells labeling ASS1 with ab191165 at 1/10 dilution (lower histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of HeLa cells labeling ASS1 with ab191165 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
ab191165 has not yet been referenced specifically in any publications.