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    ass1-antibody-epr12398-bsa-and-azide-free-ab231684.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

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Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunocytochemistry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
  • Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12398] to ASS1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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APC Conjugation Kit - Lightning-Link® (ab201807)
Knockout
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Human ASS1 knockout HeLa cell line (ab264989)
Protein
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Recombinant Human ASS1 protein (ab98084)

View more associated products

Overview

  • Product name

    Anti-ASS1 antibody [EPR12398] - BSA and Azide free
    See all ASS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12398] to ASS1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human ASS1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P00966

  • Positive control

    • WB: HAP1, HeLa, HepG2 cell lysates. Human fetal kidney and liver tissue lysates. Mouse liver and kidney lysates. Rat liver lysate. ICC/IF: MCF7 and HeLa cells. IHC-P: Human kidney, ureter tissue. Mouse kidney tissue. Flow Cyt: HeLa cells. IP: HeLa cells.
  • General notes

    ab231684 is the carrier-free version of ab170952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231684 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12398
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-ASS1 antibody [EPR12398] (ab170952)
    • Alexa Fluor® 488 Anti-ASS1 antibody [EPR12398] (ab208412)
    • HRP Anti-ASS1 antibody [EPR12398] (ab209018)
    • PE Anti-ASS1 antibody [EPR12398] (ab210451)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ASS1 knockout HeLa cell line (ab264989)
  • KO cell lysates

    • Human ASS1 knockout HeLa cell lysate (ab257143)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant Human ASS1 protein (ab98084)
  • Related Products

    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    • Recombinant Human ASS1 protein (ab98084)

Applications

Our Abpromise guarantee covers the use of ab231684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
  • Involvement in disease

    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.
  • Sequence similarities

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Target information above from: UniProt accession P00966 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280726 Cow
    • Entrez Gene: 445 Human
    • Entrez Gene: 11898 Mouse
    • Entrez Gene: 25698 Rat
    • Omim: 603470 Human
    • SwissProt: P14568 Cow
    • SwissProt: P00966 Human
    • SwissProt: P16460 Mouse
    • SwissProt: P09034 Rat
    • Unigene: 160786 Human
    • Unigene: 3217 Mouse
    • Unigene: 5078 Rat
    see all
  • Alternative names

    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

Images

  • Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    All lanes : Anti-ASS1 antibody [EPR12398] (ab170952) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ASS1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab170952).

      Lanes 1- 2: Merged signal (red and green). Green - ab170952 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab170952 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264989 (knockout cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170952 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunocytochemistry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    This data was developed using the same antibody clone in a different buffer formulation (ab170952). ab170952 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab170952 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Western blot - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    All lanes : Anti-ASS1 antibody [EPR12398] (ab170952) at 1/20000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ASS1 knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 47 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab170952 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab170952 was shown to recognize ASS1 in wild-type HAP1 cells as signal was lost at the expected MW in ASS1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. Ab170952 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/20000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling ASS1 with Purified ab170952 at 1:4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    ab170952 (purified) at 1:60 dilution (5ug) immunoprecipitating ASS1 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab170952 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ASS1 with purified ab170952 at 1:100 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling ASS1 with Purified ab170952 at 1:100 dilution (10.2μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    ab170952 showing +ve staining in Mouse kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    ab170952 showing +ve staining in Human normal ureter tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    ab170952 showing -ve staining in Human normal uterus tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    ab170952 showing -ve staining in Human normal pancreas tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    ab170952 showing -ve staining in Human cervical carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunoprecipitation - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Secondary antibody used is HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Immunofluorescent analysis of HeLa cells labeling ASS1 using ab170952 at 1/50 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ASS1 with ab170952 at 1/250 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Flow Cytometry - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Flow cytometric analysis of permeabilized Hela cells labeling ASS1 using ab170952 at 1/500 dilution  (red) or a rabbit IgG negative (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

    Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ASS1 with Purified ab170952 at 1:4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP)
    secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170952).

  • Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)
    Anti-ASS1 antibody [EPR12398] - BSA and Azide free (ab231684)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab231684? Please let us know so that we can cite the reference in this datasheet.

    ab231684 has not yet been referenced specifically in any publications.

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