Key features and details
- Rabbit polyclonal to Astrin/Deepest
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Astrin/Deepest antibody
See all Astrin/Deepest primary antibodies
DescriptionRabbit polyclonal to Astrin/Deepest
Detects endogenous levels of Astrin/Deepest protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
- HEK293T and PC12 whole cell lysate.
This product was previously labelled as Astrin
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurity is > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab200671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.|
FunctionEssential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.
Tissue specificityHighly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Chromosome > centromere > kinetochore. In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends.
- Information by UniProt
- Astrin antibody
- Deepest antibody
- hMAP 126 antibody
ab200671 has been referenced in 2 publications.
- Xu J et al. SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway. Cell Prolif 53:e12738 (2020). PubMed: 31957155
- Zhou H et al. Sperm-Associated Antigen 5 Expression Is Increased in Hepatocellular Carcinoma and Indicates Poor Prognosis. Med Sci Monit 24:6021-6028 (2018). PubMed: 30157168