Overview

  • Product name

    Anti-Astrin/Deepest antibody
    See all Astrin/Deepest primary antibodies
  • Description

    Rabbit polyclonal to Astrin/Deepest
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of Astrin/Deepest protein.

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Astrin/Deepest aa 151-196 (internal sequence).
    Sequence:

    TMAETNSISL NGPLRTDDLV REEVAPCMGD RFSEVAAVSE KPIFQE


    Database link: Q96R06

  • Positive control

    • HEK293T and PC12 whole cell lysate.
  • General notes

     This product was previously labelled as Astrin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purity is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.

Target

  • Function

    Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.
  • Tissue specificity

    Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Chromosome > centromere > kinetochore. In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends.
  • Information by UniProt
  • Database links

  • Alternative names

    • Astrin antibody
    • Deepest antibody
    • hMAP 126 antibody
    • hMAP126 antibody
    • MAP 126 antibody
    • MAP126 antibody
    • Mitotic spindle associated protein antibody
    • Mitotic spindle associated protein p126 antibody
    • Mitotic spindle coiled coil related protein antibody
    • Mitotic spindle-associated protein p126 antibody
    • SPAG 5 antibody
    • SPAG5 antibody
    • SPAG5_HUMAN antibody
    • Sperm associated antigen 5 antibody
    • Sperm tail protein Spag 5 antibody
    • Sperm tail protein Spag5 antibody
    • Sperm-associated antigen 5 antibody
    see all

Images

  • All lanes : Anti-Astrin/Deepest antibody (ab200671)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : PC12 whole cell lysate

    Predicted band size: 134 kDa

References

This product has been referenced in:

  • Zhou H  et al. Sperm-Associated Antigen 5 Expression Is Increased in Hepatocellular Carcinoma and Indicates Poor Prognosis. Med Sci Monit 24:6021-6028 (2018). Read more (PubMed: 30157168) »
See 1 Publication for this product

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