Product nameAstrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel
Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (ab226481) is an astrocyte marker panel containing 4 recombinant rabbit monoclonal antibodies and 1 Anti-Rabbit IgG (HRP) secondary antibody: Anti-GFAP antibody, Anti-ALDH1L1 antibody, Anti-EAAT1 antibody, Anti-EEAT2 antibody, and Goat Anti-Rabbit IgG (H&L) (HRP).
Astrocytes are star-shaped glial cells and serve a wide variety of functions in the central nervous system, which are vital for brain development, physiology and pathology.
The antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are available and ready to use for multiplex IHC analysis including Imaging Mass CytometryTM. Please refer to the ‘Associated products’ section below.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 packs ab177463 - Anti-ALDH1L1 antibody [EPR12743(B)] 1 x 10µl ab181036 - Anti-EAAT1 antibody [EPR12686] 1 x 10µl ab205248 - Anti-EAAT2 antibody [EPR19798] 1 x 10µl ab68428 - Anti-GFAP antibody [EPR1034Y] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H&L (HRP) 1 x 100µg
Cellular localizationGFAP: Cytoplasm. Associated with intermediate filaments. EAAT1: Membrane. EAAT2: Membrane. ALDH1L1: Cytoplasm.
- Anti-GFAP antibody [EPR1034Y] - BSA and Azide free (ab218309)
- Anti-E2F1 antibody [EPR3818(3)] - BSA and Azide free (ab218527)
- Anti-EAAT2 antibody [EPR19798] - Low endotoxin, Azide free (ab223538)
- Anti-ALDH1L1 antibody [EPR12743(B)] - BSA and Azide free (ab233016)
- Anti-EAAT1 antibody [EPR12686] - BSA and Azide free (ab240235)
Immunocytochemistry/Immunofluorescence analysis of mouse cerebellum tissue lysate labelling GFAP with ab68428 at 1/100. The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Antigen retrieval was performed by heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Nuclei were counterstained with DAPI (blue). PBS was used instead of the primary antibody as negative control.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854]. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226481 has not yet been referenced specifically in any publications.