Anti-ASUN antibody (ab227492)
Key features and details
- Rabbit polyclonal to ASUN
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ASUN antibody -
Description
Rabbit polyclonal to ASUN -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Recombinant fragment within Human ASUN (internal sequence). The exact sequence is proprietary.
Database link: Q9NVM9 -
Positive control
- ICC/IF: A431 cells.
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General notes
This product was previously labelled as C12orf11
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab227492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Crucial regulator of the mitotic cell cycle and development. At prophase, required for dynein anchoring to the nuclear envelope important for proper centrosome-nucleus coupling. At G2/M phase, may be required for proper spindle formation and execution of cytokinesis. -
Tissue specificity
Widely expressed. Tends to be up-regulated in seminomas compared to normal testis. -
Sequence similarities
Belongs to the asunder family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55726 Human
- Entrez Gene: 71177 Mouse
- Entrez Gene: 690728 Rat
- Omim: 615079 Human
- SwissProt: Q9NVM9 Human
- SwissProt: Q8QZV7 Mouse
- Unigene: 505077 Human
- Unigene: 271774 Mouse
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Alternative names
- asun antibody
- ASUN_HUMAN antibody
- Cell cycle regulator Mat89Bb homolog antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ASUN antibody (ab227492)
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for ASUN (green) using ab227492 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227492 has not yet been referenced specifically in any publications.
