Key features and details
- Rabbit polyclonal to ATAD2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATAD2 antibody
See all ATAD2 primary antibodies
DescriptionRabbit polyclonal to ATAD2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: U-2 OS cells. IHC-P: Human testis tissue. ICC/IF: U-2 OS cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 159 kDa.|
FunctionMay be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.
Tissue specificityHighly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors.
Sequence similaritiesBelongs to the AAA ATPase family.
Contains 1 bromo domain.
- Information by UniProt
- AAA nuclear coregulator cancer-associated protein antibody
- AAA nuclear coregulator, cancer-associated antibody
- ANCCA antibody
Paraffin-embedded human testis tissue stained for ATAD2 using ab244431 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human epididymis tissue stained for ATAD2 using ab244431 at 1/500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATAD2 (green) using ab244431 at 4 μg/ml in ICC/IF.
All lanes : Anti-ATAD2 antibody (ab244431) at 0.4 µg/ml
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) plus target-specific siRNA#1, cell lysate
Lane 2 : U-2 OS plus target-specific siRNA#2, cell lysate
Lane 3 : U-2 OS plus control siRNA, cell lysate
Predicted band size: 159 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244431 has not yet been referenced specifically in any publications.