Validated using a knockout cell line
Recombinant
RabMAb

Anti-ATAD2 antibody [EPR12730] (ab176319)

Overview

  • Product name
    Anti-ATAD2 antibody [EPR12730]
    See all ATAD2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12730] to ATAD2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATAD2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6PL18

  • Positive control
    • WB: HeLa, MCF-7, T47-D and Saos-2 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 159 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.
    • Tissue specificity
      Highly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors.
    • Sequence similarities
      Belongs to the AAA ATPase family.
      Contains 1 bromo domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAA nuclear coregulator cancer-associated protein antibody
      • AAA nuclear coregulator, cancer-associated antibody
      • ANCCA antibody
      • Atad2 antibody
      • ATAD2_HUMAN antibody
      • ATPase family AAA domain containing 2 antibody
      • ATPase family AAA domain containing protein 2 antibody
      • ATPase family AAA domain-containing protein 2 antibody
      • CT137 antibody
      • DKFZp667N1320 antibody
      • L16 antibody
      • MGC131938 antibody
      • MGC142216 antibody
      • MGC29843 antibody
      • MGC5254 antibody
      • PRO2000 antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ATAD2 knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab176319 was shown to specifically react with ATAD2 in wild type cells as signal was lost in ATAD2 knockout cells. Wild-type and ATAD2 knockout samples were subjected to SDS-PAGE. Ab176319 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

       

    • Immunofluorescent staining of HeLa cells labeling ATAD2 using ab176319 at a 1/100 dilution.

    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATAD2 (red) with ab176319 at a 1/60 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • All lanes : Anti-ATAD2 antibody [EPR12730] (ab176319) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : MCF-7 lysate
      Lane 3 : T47-D lysate
      Lane 4 : Saos-2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 159 kDa

    References

    ab176319 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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