Anti-Atad5 antibody (ab72111)
Key features and details
- Rabbit polyclonal to Atad5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Atad5 antibody -
Description
Rabbit polyclonal to Atad5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residues 775 and 825 of human Atad5 (NP_079133.3)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72111 was affinity purified using an epitope specific to Atad5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa).
One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell. |
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa). One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Involved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis. -
Sequence similarities
Belongs to the AAA ATPase family. -
Post-translational
modificationsATR may stimulate the RAD9A dissociation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79915 Human
- Omim: 609534 Human
- SwissProt: Q96QE3 Human
- Unigene: 528902 Human
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Alternative names
- ATAD5 antibody
- ATAD5_HUMAN antibody
- ATPase family AAA domain containing 5 antibody
see all
Images
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All lanes : Anti-Atad5 antibody (ab72111) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 208 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of Atad5 by Western Blot of Immunprecipitate.
ab72111 at 1µg/ml staining Atad5 in HeLa whole cell lysate immunoprecipitated using ab72111 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab72111 has been referenced in 3 publications.
- Wang YC et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination. Cell Rep 42:112296 (2023). PubMed: 36961817
- Shiomi Y et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol 32:2279-88 (2012). ICC/IF ; Human . PubMed: 22493068
- Rahman S et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). PubMed: 21555454