Key features and details
- Rabbit polyclonal to Atad5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Atad5 antibody
DescriptionRabbit polyclonal to Atad5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region between residues 775 and 825 of human Atad5 (NP_079133.3)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72111 was affinity purified using an epitope specific to Atad5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 208 kDa).
One of our customer have mentioned using WB protocol published in J. Biol. Chem. 2010, 285:10362-10369 with lymphoblastoid cell.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionInvolved in DNA damage response. Involved in a RAD9A-related damage checkpoint, a pathway that is important in determining whether DNA damage is compatible with cell survival or whether it requires cell elimination by apoptosis. Modulates the RAD9A interaction with BCL2 and thereby induces DNA damages-induced apoptosis.
Sequence similaritiesBelongs to the AAA ATPase family.
modificationsATR may stimulate the RAD9A dissociation.
- Information by UniProt
- ATAD5 antibody
- ATAD5_HUMAN antibody
- ATPase family AAA domain containing 5 antibody
All lanes : Anti-Atad5 antibody (ab72111) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 208 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of Atad5 by Western Blot of Immunprecipitate.
ab72111 at 1µg/ml staining Atad5 in HeLa whole cell lysate immunoprecipitated using ab72111 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab72111 has been referenced in 2 publications.
- Shiomi Y et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol 32:2279-88 (2012). ICC/IF ; Human . PubMed: 22493068
- Rahman S et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). PubMed: 21555454