Overview

  • Product name

    Anti-Ataxin 1 antibody [EPR19613]
    See all Ataxin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19613] to Ataxin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Ataxin 1 aa 550-700. The exact sequence is proprietary.
    Database link: P54253

  • Positive control

    • WB: SH-SY5Y, HeLa, U-87 MG, RAW 264.7 and Neuro-2a whole cell lysates; human cerebellum and fetal brain, kidney and heart lysates; mouse spleen, brain, heart and kidney lysates; rat brain lysate. IP: Human brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 86 kDa).
IP 1/30.

Target

  • Function

    Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.
  • Tissue specificity

    Widely expressed throughout the body.
  • Involvement in disease

    Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities

    Belongs to the ATXN1 family.
    Contains 1 AXH domain.
  • Domain

    The AXH domain is required for interaction with CIC.
  • Post-translational
    modifications

    Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
    Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
  • Cellular localization

    Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • alternative ataxin1 antibody
    • Ataxin-1 antibody
    • ATX1 antibody
    • ATX1_HUMAN antibody
    • Atxn1 antibody
    • D6S504E antibody
    • OTTHUMP00000016065 antibody
    • SCA1 antibody
    • Spinocerebellar ataxia type 1 protein antibody
    see all

Images

  • Lanes 1-5 : Anti-Ataxin 1 antibody [EPR19613] (ab201037) at 1/1000 dilution
    Lane 6 : Anti-Ataxin 1 antibody [EPR19613] (ab201037) at 1/10000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
    Lane 4 : Human cerebellum lysate
    Lane 5 : Human fetal brain lysate
    Lane 6 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 86 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,6: 3 minutes; Lane 3-5: 15 seconds.

    The molecular weight  observed is consistent with what has been described in the literature (PMID: 7647801).

  • All lanes : Anti-Ataxin 1 antibody [EPR19613] (ab201037) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution (VeriBlot for IP Detection Reaction (HRP))

    Developed using the ECL technique.

    Predicted band size: 86 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 8 seconds.

    The molecular weight  observed is consistent with what has been described in the literature (PMID: 7647801).

  • All lanes : Anti-Ataxin 1 antibody [EPR19613] (ab201037) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Rat brain lysate
    Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 86 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,5,6: 3 minutes; Lane 2-4: 8 seconds.

    The molecular weight  observed is consistent with what has been described in the literature (PMID: 7647801).

  • Ataxin 1 was immunoprecipitated from 0.35 mg of human brain lysate with ab201037 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human brain lysate 10 µg (Input). 

    Lane 2: ab201037 IP in human brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201037 in human brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab201037 has not yet been referenced specifically in any publications.

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