Overview

  • Product name

    Anti-Ataxin 1 (phospho S775) antibody
    See all Ataxin 1 primary antibodies
  • Description

    Rabbit polyclonal to Ataxin 1 (phospho S775)
  • Host species

    Rabbit
  • Specificity

    ab182638 detects endogenous levels of Ataxin 1 only when phosphorylated at Serine 775.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Ataxin 1 (phospho S775) conjugated to Keyhole Limpet Haemocyanin (KLH). (R-W-S(p)-A-P) (NP_000323.2).
    Database link: P54253

  • Positive control

    • HepG2 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride

    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab182638 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 86 kDa.

Target

  • Function

    Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function.
  • Tissue specificity

    Widely expressed throughout the body.
  • Involvement in disease

    Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities

    Belongs to the ATXN1 family.
    Contains 1 AXH domain.
  • Domain

    The AXH domain is required for interaction with CIC.
  • Post-translational
    modifications

    Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
    Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
  • Cellular localization

    Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • alternative ataxin1 antibody
    • Ataxin-1 antibody
    • ATX1 antibody
    • ATX1_HUMAN antibody
    • Atxn1 antibody
    • D6S504E antibody
    • OTTHUMP00000016065 antibody
    • SCA1 antibody
    • Spinocerebellar ataxia type 1 protein antibody
    see all

Images

  • All lanes : Anti-Ataxin 1 (phospho S775) antibody (ab182638) at 1/500 dilution

    Lane 1 : HepG2 cell lysate with immunizing peptide
    Lane 2 : HepG2 cell lysate

    Predicted band size: 86 kDa

References

ab182638 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182638.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up