Immunocytochemistry/ Immunofluorescence abreview for Anti-Ataxin 1 (phospho S776) antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK)
Specification
HEK
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.01% Triton
Blocking step
Goat Serum as blocking agent for 30 minute(s) · Concentration: 30% · Temperature: 37°C

Other product details

Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 30% Goat serum 0.01% Triton in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa-594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/400

Additional data

PubMed ID
20477910: View
Additional Notes
Image Shows: GFP-ATNX1[82Q] with intact S776 phosphorylation site and below is GFP-ATXN1[85Q] A776 with mutated S776 to alanine. Note: ATXN1 A776 mutant does not show Phospho-ATXN1 S776 staining

Image is also published in the corresponding PubMed link.

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Submitted Sep 24 2010

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