Overview

  • Product name
  • Description
    Rabbit polyclonal to Ataxin 3
  • Host species
    Rabbit
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to a sequence within the N-terminal of Human Ataxin 3 (UniProt: P54252).

  • Positive control
    • IHC-P: Human Lung Cancer Tissue; WB: Rat Brain, Rat Heart, Rat Placenta, HeLa, PANC, SMMC, COLO320 and MCF7 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab151764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1-3µg for 106 cells.

Target

  • Function
    Interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription. Acts as a deubiquitinating enzyme.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in ATXN3 are the cause of spinocerebellar ataxia type 3 (SCA3) [MIM:109150]; also known as Machado-Joseph disease (MJD). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA3 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. The molecular defect in SCA3 is the a CAG repeat expansion in ATXN3 coding region. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities
    Contains 1 Josephin domain.
    Contains 3 UIM (ubiquitin-interacting motif) repeats.
  • Cellular localization
    Nucleus matrix. Predominantly nuclear, but not exclusively, inner nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT3 antibody
    • Ataxin 3 antibody
    • ataxin 3 variant h antibody
    • ataxin 3 variant m antibody
    • ataxin 3 variant ref antibody
    • Ataxin-3 antibody
    • ATX3 antibody
    • ATX3_HUMAN antibody
    • ATXN3 antibody
    • EC 3.4.22. antibody
    • JOS antibody
    • Josephin antibody
    • Machado Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) antibody
    • Machado Joseph disease antibody
    • Machado Joseph disease protein 1 antibody
    • Machado-Joseph disease protein 1 antibody
    • Machado-Joseph disease protein 1 homolog antibody
    • MJD antibody
    • MJD gene antibody
    • MJD1 antibody
    • Olivopontocerebellar ataxia 3 antibody
    • OTTHUMP00000221583 antibody
    • OTTHUMP00000221585 antibody
    • OTTHUMP00000221586 antibody
    • OTTHUMP00000221587 antibody
    • OTTHUMP00000231995 antibody
    • OTTHUMP00000231997 antibody
    • Rsca3 antibody
    • SCA3 antibody
    • SCA3 gene antibody
    • Spinocerebellar ataxia type 3 protein antibody
    see all

Images

  • All lanes : Anti-Ataxin 3 antibody (ab151764) at 0.5 µg/ml

    Lane 1 : Rat Brain Tissue Lysate with 5% Non-fat Milk/ TBS
    Lane 2 : Rat Heart Tissue Lysate with 5% Non-fat Milk/ TBS
    Lane 3 : Human Placenta Tissue Lysate with 5% Non-fat Milk/ TBS
    Lane 4 : HeLa Whole Cell Lysate with 5% Non-fat Milk/ TBS
    Lane 5 : PANC Whole Cell Lysate with 5% Non-fat Milk/ TBS
    Lane 6 : SMMC Whole Cell Lysate with 5% Non-fat Milk/ TBS
    Lane 7 : COLO320 Whole Cell Lysate with 5% Non-fat Milk/ TBS
    Lane 8 : MCF-7 Whole Cell Lysate with 5% Non-fat Milk/ TBS

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 10000 µg/ml

    Predicted band size: 42 kDa

  • Immunohistochemical analysis of paraffin embedded Human lung cancer tissue, labeling Ataxin 3 with ab151764 at 1 μg/ml .
  • Overlay histogram showing SiHa cells stained with ab151764 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab151764 (1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

  • Overlay histogram showing MCF-7 cells stained with ab151764 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab151764 (1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

References

ab151764 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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