The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription. Acts as a deubiquitinating enzyme.
Involvement in disease
Defects in ATXN3 are the cause of spinocerebellar ataxia type 3 (SCA3) [MIM:109150]; also known as Machado-Joseph disease (MJD). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA3 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. The molecular defect in SCA3 is the a CAG repeat expansion in ATXN3 coding region. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
Immunohistochemical analysis of paraffin embedded Human lung cancer tissue, labeling Ataxin 3 with ab151764 at 1 μg/ml .
Western blot - Anti-Ataxin 3 antibody (ab151764)
All lanes : Anti-Ataxin 3 antibody (ab151764) at 0.5 µg/ml
Lane 1 : Rat Brain Tissue Lysate Lane 2 : Rat Heart Tissue Lysate Lane 3 : Rat Placenta Tissue Lysate Lane 4 : HeLa Cell Lysate Lane 5 : PANC Cell Lysate Lane 6 : SMMC Cell Lysate Lane 7 : COLO320 Cell Lysate Lane 8 : MCF7 Cell Lysate