Key features and details
- Rabbit polyclonal to Ataxin 3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Ataxin 3 antibody
See all Ataxin 3 primary antibodies
DescriptionRabbit polyclonal to Ataxin 3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Ataxin 3 aa 1-364.
MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAE GGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQ RLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLAL FLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLK EQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLR RAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQ QQQQQGDLSGQSSHPCERPATSSGALGSDLGKACSPFIMFATFTLYLTYE LHVIFALHYSSFPL
Database link: P54252
- MCF7 whole cell lysate (ab29537) can be used as a positive control in WB. MCF7 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 42 kDa.|
FunctionInteracts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription. Acts as a deubiquitinating enzyme.
Involvement in diseaseDefects in ATXN3 are the cause of spinocerebellar ataxia type 3 (SCA3) [MIM:109150]; also known as Machado-Joseph disease (MJD). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA3 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. The molecular defect in SCA3 is the a CAG repeat expansion in ATXN3 coding region. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
Sequence similaritiesContains 1 Josephin domain.
Contains 3 UIM (ubiquitin-interacting motif) repeats.
Cellular localizationNucleus matrix. Predominantly nuclear, but not exclusively, inner nuclear matrix.
- Information by UniProt
- AT3 antibody
- Ataxin 3 antibody
- ataxin 3 variant h antibody
Anti-Ataxin 3 antibody (ab175265) at 1/500 dilution + MCF7 cell lysate
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling Ataxin 3 with ab175265 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling Ataxin 3 with ab175265 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175265. Blue DAPI for nuclear staining.
ab175265 has been referenced in 1 publication.
- Ouyang S et al. CRISPR/Cas9-Targeted Deletion of Polyglutamine in Spinocerebellar Ataxia Type 3-Derived Induced Pluripotent Stem Cells. Stem Cells Dev 27:756-770 (2018). PubMed: 29661116