Recombinant Anti-Ataxin 3 antibody [EPR22418-147] (ab221143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22418-147] to Ataxin 3
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ataxin 3 antibody [EPR22418-147]
See all Ataxin 3 primary antibodies -
Description
Rabbit monoclonal [EPR22418-147] to Ataxin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Indirect ELISAmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-87 MG, NIH/3T3 and C6 whole cell lysate; Human brain, spleen and cerebellum tissue lysate; Mouse skin and spleen tissue lysate; Rat brain and cerebellum tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22418-147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 35, 45 kDa (predicted molecular weight: 42 kDa).
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Indirect ELISA |
Use a concentration of 1 µg/ml.
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Notes |
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WB
1/1000. Detects a band of approximately 35, 45 kDa (predicted molecular weight: 42 kDa). |
Indirect ELISA
Use a concentration of 1 µg/ml. |
Target
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Function
Interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription. Acts as a histone-binding protein that regulates transcription. Acts as a deubiquitinating enzyme. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in ATXN3 are the cause of spinocerebellar ataxia type 3 (SCA3) [MIM:109150]; also known as Machado-Joseph disease (MJD). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA3 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. The molecular defect in SCA3 is the a CAG repeat expansion in ATXN3 coding region. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Contains 1 Josephin domain.
Contains 3 UIM (ubiquitin-interacting motif) repeats. -
Cellular localization
Nucleus matrix. Predominantly nuclear, but not exclusively, inner nuclear matrix. - Information by UniProt
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Database links
- Entrez Gene: 4287 Human
- Entrez Gene: 110616 Mouse
- Entrez Gene: 60331 Rat
- Omim: 607047 Human
- SwissProt: P54252 Human
- SwissProt: Q9CVD2 Mouse
- SwissProt: O35815 Rat
- Unigene: 532632 Human
see all -
Alternative names
- AT3 antibody
- Ataxin 3 antibody
- ataxin 3 variant h antibody
see all
Images
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All lanes : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 4 : Mouse skin tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Rat brain tissue lysate
Lane 7 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-2: 26 seconds
Lanes 3-4: 3 minutes
Lane 5: 15 seconds
Lane 6: 3 minutes
Lane 7: 59 seconds
The molecular weight is consistent with what has been described in the literature (PMID: 30455355).
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Indirect ELISA using ab221143 at varying antibody concentrations (1000-0 ng/ml) and Human ATXN3 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.
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Lanes 1-3 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/1000 dilution
Lane 4 : Anti-Ataxin 3 antibody [EPR22418-147] (ab221143) at 1/5000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Human cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 45, 35 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lane 1: 10 seconds
Lanes 2-3: 125 seconds
Lane 4: 5 seconds
The full-length ataxin-3 (45kDa) and a 35-kDa band, likely to be an ataxin-3 isoform or fragment, are observed. The molecular weights are consistent with what have been described in the literature (PMID: 30455355 and UniProt annotation).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab221143 has not yet been referenced specifically in any publications.