Recombinant Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)
![Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-447668-anti-ataxin7antibody-epr2413689-western-blot-lysate.jpg "Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24136-89] to Ataxin 7
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Ataxin 7 antibody [EPR24136-89]
See all Ataxin 7 primary antibodies -
Description
Rabbit monoclonal [EPR24136-89] to Ataxin 7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse Ataxin 7 aa 50-150. The exact sequence is proprietary.
Database link: Q8R4I1 -
Positive control
- WB: Mouse brain tissue lysate; Neuro-2a whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24136-89 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab259829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa). |
Target
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Relevance
Ataxin 7 is a protein of unknown function. It may be the human orthologue of the yeast SAGA SGF73 subunit and a subunit of the human TFTC-like transcriptional complexes. Spinocerebellar ataxia 7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded trinucleotide repeat in the gene encoding ataxin 7. Ataxin 7 is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death. -
Cellular localization
Cytoplasmic (isoform b) and Nuclear (isoform a) -
Database links
- Entrez Gene: 246103 Mouse
- SwissProt: Q8R4I1 Mouse
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Alternative names
- ADCAII antibody
- ATXN 7 antibody
- OPCA III antibody
see all
Images
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Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)All lanes : Anti-Ataxin 7 antibody [EPR24136-89] (ab259829) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The MW is consistent to literature (PMID:22367614).
Negative control: mouse kidney (PMID:11099453).
Exposure time: 81 seconds.
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![Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-447669-anti-ataxin7antibody-epr2413689-western-blot-neuroblast.jpg)
Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)Anti-Ataxin 7 antibody [EPR24136-89] (ab259829) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
WB positive cell line: Neuro-2a (PMID:25306109)
Exposure time: 125 seconds.
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![Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)](https://www.abcam.com/ps/products/259/ab259829/Images/ab259829-448067-benefitsofrecombinantantibodies.png)
Anti-Ataxin 7 antibody [EPR24136-89] (ab259829)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab259829 has not yet been referenced specifically in any publications.
