Recombinant Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13667(2)] to ATE1 - N-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ATE1 antibody [EPR13667(2)] - N-terminal
See all ATE1 primary antibodies -
Description
Rabbit monoclonal [EPR13667(2)] to ATE1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa and 293 whole cell lysate. Human fetal liver tissue lysate. Mouse brain and spleen tissue lysate. C6, Raw264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and Rat kidney tissue lysate. Rat spleen tissue lysate. IHC: Mouse liver tissue, Human hepatocellular carcinoma tissue, Rat kidney tissue. ICC/IF: HepG2 cells. Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13667(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/220.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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Notes |
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Flow Cyt (Intra)
1/220. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues. -
Sequence similarities
Belongs to the R-transferase family. -
Cellular localization
Cytoplasm and Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11101 Human
- Entrez Gene: 11907 Mouse
- Entrez Gene: 293526 Rat
- Omim: 607103 Human
- SwissProt: O95260 Human
- SwissProt: Q9Z2A5 Mouse
- Unigene: 632080 Human
- Unigene: 216321 Mouse
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Alternative names
- AI225793 antibody
- Arginine tRNA protein transferase 1 antibody
- Arginine-tRNA--protein transferase 1 antibody
see all
Images
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All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
Lane 5 : Mouse kidney tissue lysate
Lane 6 : Rat kidney tissue lysate
Lane 7 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutesBlocking and diluting buffer 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasmic staining on human hepatocellular carcinoma tissue is observed (Subcellular location: Nucleus and cytoplasm [UniProt]). Counter-stained with hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATE1 with ab199423 at 1/220 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 3 minutesBlocking and diluting buffer 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 1 minuteBlocking and diluting buffer 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Rat kidney tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 10 secondsBlocking and diluting buffer 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab199423 has been referenced in 1 publication.
- Singh K et al. Arginyltransferase knockdown attenuates cardiac hypertrophy and fibrosis through TAK1-JNK1/2 pathway. Sci Rep 10:598 (2020). PubMed: 31953451