Recombinant
RabMAb

Recombinant Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

Overview

  • Product name

    Anti-ATE1 antibody [EPR13667(2)] - N-terminal
    See all ATE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13667(2)] to ATE1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATE1 aa 1-100. The exact sequence is proprietary.
    Database link: O95260

  • Positive control

    • WB: HepG2, HeLa and 293 whole cell lysate. Human fetal liver tissue lysate. Mouse brain and spleen tissue lysate. C6, Raw264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and Rat kidney tissue lysate. Rat spleen tissue lysate. IHC: Mouse liver tissue, Human hepatocellular carcinoma tissue, Rat kidney tissue. ICC/IF: HepG2 cells. FC: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13667(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/250.
Flow Cyt 1/220.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.
  • Sequence similarities

    Belongs to the R-transferase family.
  • Cellular localization

    Cytoplasm and Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI225793 antibody
    • Arginine tRNA protein transferase 1 antibody
    • Arginine-tRNA--protein transferase 1 antibody
    • Arginyl-tRNA--protein transferase 1 antibody
    • Arginyl-tRNA-protein transferase antibody
    • Arginyltransferase 1 antibody
    • Ate1 antibody
    • ATE1_HUMAN antibody
    • AW547406 antibody
    • EC 2.3.2.8 antibody
    • MGC26724 antibody
    • OTTHUMP00000020637 antibody
    • OTTHUMP00000058665 antibody
    • R transferase 1 antibody
    • R-transferase 1 antibody
    see all

Images

  • All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasmic staining on human hepatocellular carcinoma tissue is observed (Subcellular location: Nucleus and cytoplasm [UniProt]). Counter-stained with hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATE1 with ab199423 at 1/220 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
    Lane 3 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 1 minute


    Blocking and diluting buffer 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Cytoplasm staining on Rat kidney tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution + Human fetal liver tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer 5% NFDM/TBST

References

ab199423 has not yet been referenced specifically in any publications.

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