- Datasheet
- Specific References (12)
- Protocols
Overview
-
Product nameAnti-ATF-4 antibody
See all ATF-4 primary antibodies -
DescriptionRabbit polyclonal to ATF-4
-
Host speciesRabbit
-
Tested applicationsSuitable for: ICC/IF, WBmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
-
Positive control
- ATF-4 tagged recombinant protein This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab23760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa). |
Target
-
FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
-
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
- Information by UniProt
-
Database links
- Entrez Gene: 468 Human
- Entrez Gene: 11911 Mouse
- Entrez Gene: 79255 Rat
- Omim: 604064 Human
- SwissProt: P18848 Human
- SwissProt: Q06507 Mouse
- SwissProt: Q9ES19 Rat
- Unigene: 496487 Human
see all -
Alternative names
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
see all
Images
-
Western blot - Anti-ATF-4 antibody (ab23760)Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 76 kDa (why is the actual band size different from the predicted?)
Ab23760 gave a positive signal against the tagged recombinant ATF4 protein which is known to run at 76 kDa. -
Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Wang Y et al. GCN2 deficiency ameliorates doxorubicin-induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress. Redox Biol 17:25-34 (2018). Read more (PubMed: 29660505) »
- Sharon E & Frenkel N Human Herpesvirus 6A Exhibits Restrictive Propagation with Limited Activation of the Protein Kinase R-eIF2a Stress Pathway. J Virol 91:N/A (2017). Read more (PubMed: 28202752) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"