For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    atf-4-antibody-ab23760.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
Share by email

Anti-ATF-4 antibody (ab23760)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (26)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-ATF-4 antibody (ab23760)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)

Key features and details

  • Rabbit polyclonal to ATF-4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ATF-4 protein (ab109946)
Primary
Product image
Anti-eIF2A antibody [EPR11042] (ab169528)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ATF-4 antibody
    See all ATF-4 primary antibodies
  • Description

    Rabbit polyclonal to ATF-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P18848
    (Peptide available as ab24034)

  • Positive control

    • ATF-4 tagged recombinant protein This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ATF-4 protein (ab109946)

Applications

Our Abpromise guarantee covers the use of ab23760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Target information above from: UniProt accession P18848 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 468 Human
    • Entrez Gene: 11911 Mouse
    • Entrez Gene: 79255 Rat
    • Omim: 604064 Human
    • SwissProt: P18848 Human
    • SwissProt: Q06507 Mouse
    • SwissProt: Q9ES19 Rat
    • Unigene: 496487 Human
    • Unigene: 641 Mouse
    • Unigene: 2423 Rat
    see all
  • Alternative names

    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • Western blot - Anti-ATF-4 antibody (ab23760)
    Western blot - Anti-ATF-4 antibody (ab23760)
    Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    Ab23760 gave a positive signal against the tagged recombinant ATF4 protein which is known to run at 76 kDa.
  • Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)
    Immunocytochemistry/ Immunofluorescence - Anti-ATF-4 antibody (ab23760)

    ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (26)

    Publishing research using ab23760? Please let us know so that we can cite the reference in this datasheet.

    ab23760 has been referenced in 26 publications.

    • Zhu X  et al. lncRNA MEG3 promotes hepatic insulin resistance by serving as a competing endogenous RNA of miR-214 to regulate ATF4 expression. Int J Mol Med 43:345-357 (2019). PubMed: 30431065
    • Lin J  et al. Berberine, a Traditional Chinese Medicine, Reduces Inflammation in Adipose Tissue, Polarizes M2 Macrophages, and Increases Energy Expenditure in Mice Fed a High-Fat Diet. Med Sci Monit 25:87-97 (2019). PubMed: 30606998
    • Huo JF & Chen XB P2X4R silence suppresses glioma cell growth through BDNF/TrkB/ATF4 signaling pathway. J Cell Biochem 120:6322-6329 (2019). PubMed: 30362154
    • Cui Y  et al. Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2-PERK pathway. Int J Mol Med 43:2015-2023 (2019). PubMed: 30864682
    • Zhou XT  et al. Inhibition of autophagy enhances adenosine-induced apoptosis in human hepatoblastoma HepG2 cells. Oncol Rep 41:829-838 (2019). PubMed: 30535464
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Question

    Please could you let us know when we should receive the antibodies in substitution?   Thank you very much

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2011

    Answer

    I have received confirmation that these 3 replacement vials will be included in the next shipment to Aurogene. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 29 2011

    Question

    our customer told us that a control can not solve her problems. It could be useful only for the difference between the expected and observed molecular weight (in our case relative to RANKL antibody), but also in that case she's sure that there was no problem in gel running and the molecular weights were indeed those identified by the standard, because after revealing RANKL they have also revealed actin, with the band showing the expected length. Moreover, your the use of a control does not resolve the problem of OPG and ATF4 aspecificity. For this reason, she has requested the substitution of items ab10962, ab23760, ab45039. Please could you let us know when you should ship us these antibodies in substitution? Our customer needs them urgently.  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 28 2011

    Answer

    Thanks for your reply. It seems that, as part of their own internal QC, the customer has already peformed the control I suggested. This is great as it does appear clear now that these 3 antibodies are not working as expected. I would be happy to request the replacement antibodies the customer asked for: 1 vial each of ab10962, ab23760, ab45039. So I can process the request, please let me know the purchase order number or Abcam order reference number associated with the initial order of these 3 antibodies. Thanks!

    Read More

    Abcam Scientific Support

    Answered on Nov 28 2011

    Question

    What species of bone cells were being tested?   Murine Osteoblasts from calvaria What dilution of primary antibody for each?   OPG 1:100; ATF4 1:400; RANKL 1:500 Was any other antibody used successfully with these same samples?  No How much protein was loaded on the gel? 20 ug   Please, could you tell us when you'll ship us the antibodies in substitution?  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 16 2011

    Answer

    Thank you for providing the additional information. The one thing I would like to recommend in this case is to run a "loading control" western blot with the murine osteoblast cell lysate. Examples of targets for this type of control would be proteins that are commonly expressed at high levels in all cell types, such as actin or GAPDH. This would serve as a control in that detection of the expected band size would indicate that all aspects of the lysate preparation and western blot protocol are working as expected. Thanks so much for your troubleshooting efforts! I look forward to hearing how things go.

    Read More

    Abcam Scientific Support

    Answered on Nov 16 2011

    Question

    the last June our company Aurogene ordered from you the antibodies ab10962 goat polyclonal to Osteoprotegerin, ab23760 rabbit polyclonal to ATF4 and ab45039 mouse monoclonal |12A668| ro RANKL, that didn't work when used by our customer. In attachment we send you the WB images of these antibodies. The experiments were performed using lysates of bone cells that certainly synthesize proteins for which the above antibodies should be specific. As for the first 2, the customer has found a strong aspecificity, while for RANKL he has seen a single band, but the found molecular weight (65 kDa approximately) is much higher than expected (35 kDa). Please, could you substitute as soon as possible these antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2011

    Answer

     Thank you for your enquiry. Sorry to hear your customer's are having trouble with these antibodies.   What species of bone cells were being tested? What dilution of primary antibody for each? Was any other antibody used successfully with these same samples? How much protein was loaded on the gel? What was the purchase order number or Abcam order reference number associated with these purchases? Thanks in advance for the additional information.

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2011

    Question

    BATCH NUMBER 158060 ORDER NUMBER 148114 DESCRIPTION OF THE PROBLEM No signal SAMPLE cell extract PRIMARY ANTIBODY Abcam/Rabbit plyclone to ATF-4/1ugm/ml / O/N incubation at 4C DETECTION METHOD ECLPlus POSITIVE AND NEGATIVE CONTROLS USED Positive Control Liver Extract SAMPLE PREPARATION Buffer AMOUNT OF PROTEIN LOADED 50-100 ugm/lane ELECTROPHORESIS/GEL CONDITIONS 10% PAGE gel TRANSFER AND BLOCKING CONDITIONS PBST 5% Non fat Milk SECONDARY ANTIBODY [a competitor]/anti Rabbitdilution is 1:5000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I tried 1 hour blocking and O/N with primary ab/ 1hour incubation and 1 hr pr. ab. at 4C and Room temp. and also change the blocking buffer dried milk to BSA with and without Twin-20

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2006

    Answer

    Thank you for contacting us for technical support and for taking the time to provide your protocol information. I'm sorry to hear you are experiencing problems with this antibody. I have tried to find in the literature references for the presence of ATF4 in liver and have not been successful, you mention it as a positive control and I would be very grateful if you could confirm the source of this information as I currently think that levels in the liver may be low. SwissProt mentions lung and placenta as tissues expressing the protein, and we have used HeLa lysate as our positive control. I would recommend to run this lysate along your samples to make sure this is not the problem. I would also like to recommend to perform a nuclear extraction procedure and to make sure the extraction buffer you currently use is strong enough to break the nucleus, as the protein is there. Could you please also check that the problem is not due to the secondary antibody, and can I check that you have been using reducing conditions? I hope the above recommendations are useful, please do not hesitate to contact me if you still experience problems with the positive control of Hela lysate,

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.