Product nameAnti-ATF-4 antibody
See all ATF-4 primary antibodies
DescriptionRabbit polyclonal to ATF-4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- ATF-4 tagged recombinant protein This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab23760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).|
FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
- Information by UniProt
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
Anti-ATF-4 antibody (ab23760) at 1 µg/ml + ATF4 tagged recombinant protein at 0.1 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Ab23760 gave a positive signal against the tagged recombinant ATF4 protein which is known to run at 76 kDa.
ICC/IF image of ab23760 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab23760 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Zhu X et al. lncRNA MEG3 promotes hepatic insulin resistance by serving as a competing endogenous RNA of miR-214 to regulate ATF4 expression. Int J Mol Med 43:345-357 (2019). Read more (PubMed: 30431065) »
- Yang F et al. ER-stress regulates macrophage polarization through pancreatic EIF-2alpha kinase. Cell Immunol N/A:N/A (2018). Read more (PubMed: 30594305) »