Overview

  • Product name

  • Description

    Rabbit polyclonal to ATF-4
  • Host species

    Rabbit
  • Specificity

    Antibody detects endogenous levels of ATF-4 protein around Serine 245.
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • Positive control

    • Breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab31390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21933012Fixed with methanol
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function

    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • ab31390 at a 1:50-1:100 dilution staining ATF-4 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : Un-treated.

    Right image : Antibody pre-incubated with synthesized peptide.

References

This product has been referenced in:

  • Liu P  et al. Crizotinib-induced immunogenic cell death in non-small cell lung cancer. Nat Commun 10:1486 (2019). Read more (PubMed: 30940805) »
  • Zheng C  et al. Suppressing UPR-dependent overactivation of FGFR3 signaling ameliorates SLC26A2-deficient chondrodysplasias. EBioMedicine 40:695-709 (2019). Read more (PubMed: 30685387) »
See all 7 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Mar 14 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast carcinoma xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
breast carcinoma xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Mar 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary rat cortical neurons)
Permeabilization
Yes - 0.1% Triton-x
Specification
Primary rat cortical neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 30 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C

Dr. Carl Atkinson

Verified customer

Submitted Apr 06 2013

Answer

Thank you for contacting us.

For ab21685 we used human liver carcinoma. I would suggest using the following product as a positive control:
Liver tumor (human): hepatocellular carcinoma tissue slides (ab4877) (https://www.abcam.com/ab4877).

For ab31390 we used ductal breast carcinoma tissue so I would suggest the following product from our catalogue:
Breast tumor (human): ductal carcinoma (invasive) tissue slides (ab4697) (https://www.abcam.com/ab4697)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you very much for your kind reply. Indeed, ab50546 has passed our strict quality controls and we have received no complaints about this product, therefore, we assume that the individual vial you had received was faulty which may happen occasionally.  Please be reassured that the replacement is also covered by our Abpromise and we will be pleased to provide a credit note, free of charge replacement or refund if it is not working as expected.  I wish you the best of luck for the submission of your paper! Have a great day!

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this ATF4 antibody. The details you have kindly provided will provide us with vital information for our monitoring of product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.   As requested, I have issued you a free of charge replacement with ab50546 with the order number of xxxxxx. To check the status of your order please contact our Customer Service team and reference this number. I apologize for the inconvenience this has caused. Please do not hesitate to contact us if you have any further questions or concerns.  

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our ATF4 antibody ab50546. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. We have received no complaints about this product therefore I am concerned it is not working for you. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records. In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I would appreciate if you could also provide an image which would help us to assess the results. We will happily offer technical support and in the event that the product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note, free of charge replacement or refund, as you purchased it just last week. Please be reassured that I will process your case immediately and if necessary I can send you ab31390 at once as we currently have it in stock.   Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.  

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