Anti-ATF-4 antibody (ab31390)
Key features and details
- Rabbit polyclonal to ATF-4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATF-4 antibody
See all ATF-4 primary antibodies -
Description
Rabbit polyclonal to ATF-4 -
Host species
Rabbit -
Specificity
Antibody detects endogenous levels of ATF-4 protein around Serine 245. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ATF-4.
Database link: P18848 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Corresponding phospho antibody
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab31390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes. - Information by UniProt
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Database links
- Entrez Gene: 468 Human
- Omim: 604064 Human
- SwissProt: P18848 Human
- Unigene: 496487 Human
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Alternative names
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 antibody (ab31390)
ab31390 at a 1:50-1:100 dilution staining ATF-4 in human breast carcinoma tissue, using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : Un-treated.
Right image : Antibody pre-incubated with synthesized peptide.
Protocols
Datasheets and documents
References (7)
ab31390 has been referenced in 7 publications.
- Liu P et al. Crizotinib-induced immunogenic cell death in non-small cell lung cancer. Nat Commun 10:1486 (2019). PubMed: 30940805
- Zheng C et al. Suppressing UPR-dependent overactivation of FGFR3 signaling ameliorates SLC26A2-deficient chondrodysplasias. EBioMedicine 40:695-709 (2019). PubMed: 30685387
- Zhu H et al. Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients. PLoS One 9:e103882 (2014). IHC-P ; Human . PubMed: 25078779
- Lenna S et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013). IHC-P ; Human . PubMed: 23441162
- Ohri SS et al. Attenuating the endoplasmic reticulum stress response improves functional recovery after spinal cord injury. Glia 59:1489-502 (2011). IHC ; Mouse . PubMed: 21638341
- Ohri SS et al. Deletion of the pro-apoptotic endoplasmic reticulum stress response effector CHOP does not result in improved locomotor function after severe contusive spinal cord injury. J Neurotrauma : (2011). ICC/IF ; Mouse . PubMed: 21933012
- Ameri K et al. Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. Br J Cancer 102:561-9 (2010). IHC-P ; Human . PubMed: 20051957
