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I’m trying to optimize immunohistochemistry protocols for the two following antibodies: Anti-ATF4 (ab31390) and Anti-GRP78 BiP (ab21685).
Your product sheets mention liver and breast carcinomas as positive controls for IHC-P. Could you give me more details as to what sort of carcinomas would be best?
Asked on Nov 28 2012
Thank you for contacting us.
For ab21685 we used human liver carcinoma. I would suggest using the following product as a positive control:
Liver tumor (human): hepatocellular carcinoma tissue slides (ab4877) (https://www.abcam.com/ab4877).
For ab31390 we used ductal breast carcinoma tissue so I would suggest the following product from our catalogue:
Breast tumor (human): ductal carcinoma (invasive) tissue slides (ab4697) (https://www.abcam.com/ab4697)
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447
Answered on Nov 28 2012