Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary rat cortical neurons)
Permeabilization
Yes - 0.1% Triton-x
Specification
Primary rat cortical neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% serum in PBS
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Treated with tunicamycin (5-ug/mL) for 24 hr
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in rat, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in rat which will help us assess how well the antibody works in this species.
Abcam user community
Verified customer
Submitted May 30 2018