Human Predicted to work with:
Synthetic peptide corresponding to Human ATF-4 (C terminal). Database link: P18848
WB: HeLa cell lysates.
Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Use at an assay dependent concentration.
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Belongs to the bZIP family. Contains 1 bZIP domain.
Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
Evstafieva AG et al. Implication of KRT16, FAM129A and HKDC1 genes as ATF4 regulated components of the integrated stress response. PLoS One13:e0191107 (2018).
Read more (PubMed: 29420561) »
Zheng S et al. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol195:217-26 (2015).
Read more (PubMed: 26019273) »