Product nameAnti-ATF-4 antibody [N360A/24]
See all ATF-4 primary antibodies
DescriptionMouse monoclonal [N360A/24] to ATF-4
Specificityab186284 has <50% identity with ATF5, it does not cross-react with this protein.
Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human ATF-4 aa 25-327.
LGAEESLGLLDDYLEVAKHFKPHGFSSDKAKAGSSEWLAVDGLVSPSNNS KEDAFSGTDWMLEKMDLKEFDLDALLGIDDLETMPDDLLTTLDDTCDLFA PLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFLQPLPLSPGVLSSTP DHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDNDSGICMS PESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVK GEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERA DSL
Database link: P18848
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S360A-24 to N360A/24, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
- Information by UniProt
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
SK-N-BE (Human neuroblastoma cell line) cells stained for ATF-4 (Green) using ab186284 at a 1/100 dilution (1 hour at RT) in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Anti-ATF-4 antibody [N360A/24] (ab186284) at 1/2000 dilution + Rat brain lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 5 minutes
Block: 5% Skimmed milk in 1X TBST.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
ab186284 has not yet been referenced specifically in any publications.