Overview

  • Product name
    Anti-ATF-4 (phospho S245) antibody
    See all ATF-4 primary antibodies
  • Description
    Rabbit polyclonal to ATF-4 (phospho S245)
  • Host species
    Rabbit
  • Specificity
    ab28830 recognizes Phospho-ATF-4(Ser245).
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab28830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating transcription factor 4 antibody
    • ATF 4 antibody
    • ATF4 antibody
    • ATF4 protein antibody
    • ATF4_HUMAN antibody
    • cAMP-dependent transcription factor ATF-4 antibody
    • cAMP-responsive element-binding protein 2 antibody
    • CREB 2 antibody
    • CREB-2 antibody
    • CREB2 antibody
    • Cyclic AMP dependent transcription factor ATF 4 antibody
    • Cyclic AMP response element binding protein 2 antibody
    • Cyclic AMP-dependent transcription factor ATF-4 antibody
    • Cyclic AMP-responsive element-binding protein 2 antibody
    • DNA binding protein TAXREB67 antibody
    • DNA-binding protein TAXREB67 antibody
    • Tax Responsive Enhancer Element B67 antibody
    • Tax-responsive enhancer element-binding protein 67 antibody
    • TaxREB67 antibody
    • TXREB antibody
    see all

Images

  • ab28830 staining ATF-4 (phospho S245) in Human MRC5 cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830
  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830 treated with synthesised phospho-peptide.

References

This product has been referenced in:
  • El-Khattouti A  et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-?B inhibition. J Cell Mol Med 20:266-86 (2016). WB . Read more (PubMed: 26578344) »
  • Kalamida D  et al. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS One 9:e102408 (2014). WB ; Human . Read more (PubMed: 25010689) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (MRC5)
Specification
MRC5
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde

Dr. Dimitra Kalamida

Verified customer

Submitted Dec 12 2013

Answer

Thank you for your inquiry. I am happy to confirm that we have a variety of antibodies against targets that are considered related with ER stress: ATF6, bip, Calnexin, IRE1a, PDI, CHOP, Perk, Ero1 Lalpha To find these antibodies please type in the name of the target in the search bar on our homepage. If you would like further help to decide which antibody to choose, please provide some additional information about the application and species you are using. I can also confirm that we have an ATF4 phospho Antibody, ab28830: Click here (or use the following: https://www.abcam.com/index.html?datasheet=28830). This antibody is tested and guaranteed for IHC-P and ELISA in human samples. If your application or species is not stated as tested on the datasheet you might be eligible for our testing discount program. I hope this information is helpful. Please do not hesitate to contact us with any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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