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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-ATF-4 (phospho S245) antibody (ab28830)

  • Datasheet
Reviews (1)Q&A (1)References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)

Key features and details

  • Rabbit polyclonal to ATF-4 (phospho S245)
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ATF-4 protein (ab109946)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ATF-4 (phospho S245) antibody
    See all ATF-4 primary antibodies
  • Description

    Rabbit polyclonal to ATF-4 (phospho S245)
  • Host species

    Rabbit
  • Specificity

    ab28830 recognizes Phospho-ATF-4(Ser245).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ATF-4.
    Database link: P18848

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Corresponding non-phospho antibody

    • Anti-ATF-4 antibody (ab31390)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human ATF-4 protein (ab109946)

Applications

Our Abpromise guarantee covers the use of ab28830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Target

  • Function

    Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
  • Target information above from: UniProt accession P18848 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 468 Human
    • Omim: 604064 Human
    • SwissProt: P18848 Human
    • Unigene: 496487 Human
    • Alternative names

      • Activating transcription factor 4 antibody
      • ATF 4 antibody
      • ATF4 antibody
      • ATF4 protein antibody
      • ATF4_HUMAN antibody
      • cAMP-dependent transcription factor ATF-4 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • Cyclic AMP dependent transcription factor ATF 4 antibody
      • Cyclic AMP response element binding protein 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-4 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • DNA binding protein TAXREB67 antibody
      • DNA-binding protein TAXREB67 antibody
      • Tax Responsive Enhancer Element B67 antibody
      • Tax-responsive enhancer element-binding protein 67 antibody
      • TaxREB67 antibody
      • TXREB antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)
      Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF-4 (phospho S245) antibody (ab28830)
      Immunohistochemistry (Formalin-fixed paraffin-embedded sections) staining of breast carcinoma using ab28830 treated with synthesised phospho-peptide.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab28830? Please let us know so that we can cite the reference in this datasheet.

    ab28830 has been referenced in 4 publications.

    • González-González A  et al. Activating Transcription Factor 4 Modulates TGFß-Induced Aggressiveness in Triple-Negative Breast Cancer via SMAD2/3/4 and mTORC2 Signaling. Clin Cancer Res 24:5697-5709 (2018). PubMed: 30012564
    • El-Khattouti A  et al. Imiquimod-induced apoptosis of melanoma cells is mediated by ER stress-dependent Noxa induction and enhanced by NF-?B inhibition. J Cell Mol Med 20:266-86 (2016). WB . PubMed: 26578344
    • Kalamida D  et al. Important role of autophagy in endothelial cell response to ionizing radiation. PLoS One 9:e102408 (2014). WB ; Human . PubMed: 25010689
    • Myers SA  et al. CD36 deletion improves recovery from spinal cord injury. Exp Neurol 256:25-38 (2014). PubMed: 24690303

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

       information regarding ER stress antibodies and phospho atf4 antibodies.   Best Regards,  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Thank you for your inquiry. I am happy to confirm that we have a variety of antibodies against targets that are considered related with ER stress: ATF6, bip, Calnexin, IRE1a, PDI, CHOP, Perk, Ero1 Lalpha To find these antibodies please type in the name of the target in the search bar on our homepage. If you would like further help to decide which antibody to choose, please provide some additional information about the application and species you are using. I can also confirm that we have an ATF4 phospho Antibody, ab28830: Click here (or use the following: https://www.abcam.com/ATF-4-phospho-S245-antibody-ab28830.html). This antibody is tested and guaranteed for IHC-P and ELISA in human samples. If your application or species is not stated as tested on the datasheet you might be eligible for our testing discount program. I hope this information is helpful. Please do not hesitate to contact us with any further questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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