Overview

  • Product name

  • Description

    Rabbit polyclonal to ATF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATF1 aa 1-50. The exact sequence is proprietary. (NP_005162.1).
    Database link: P18846

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue. WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225880 was affinity purified using an epitope specific to ATF1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 29 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
  • Involvement in disease

    Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor 1 antibody
    • ATF 1 antibody
    • atf1 antibody
    • ATF1 EWS fusion gene antibody
    • ATF1 FUS fusion gene antibody
    • ATF1_HUMAN antibody
    • cAMP dependent transcription factor 1 antibody
    • cAMP-dependent transcription factor ATF-1 antibody
    • Cyclic AMP dependent transcription factor ATF 1 antibody
    • Cyclic AMP dependent transcription factor ATF1 antibody
    • Cyclic AMP-dependent transcription factor ATF-1 antibody
    • EWS AFT1 antibody
    • FUS ATF 1 antibody
    • FUS/ATF 1 antibody
    • Protein TREB36 antibody
    • RNA binding protein activating transcription factor 1 fusion protein antibody
    • TREB 36 antibody
    • TREB36 antibody
    • TREB36 protein antibody
    see all

Images

  • All lanes : Anti-ATF1 antibody (ab225880) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for ATF1 using ab225880 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

  • ATF1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab225880 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab225880 at 0.4 µg/ml.

    Lane 1: ab225880 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab225880 has not yet been referenced specifically in any publications.

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