Key features and details
- Rabbit polyclonal to ATF1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-ATF1 antibody
See all ATF1 primary antibodies
DescriptionRabbit polyclonal to ATF1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Cow
Recombinant fragment (His-T7-tag) corresponding to Human ATF1 aa 3-213. (Expressed in E.coli).
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEF-DSHKSTTSETAPQ PGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYR KILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPN GALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSN QVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQ
Database link: P18846
- WB: Recombinant human ATF1 protein, Rat and Porcine serum. IHC-P: Human ovary tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231527 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.|
FunctionThis protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
Involvement in diseaseDefects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
- Information by UniProt
- Activating transcription factor 1 antibody
- ATF 1 antibody
- atf1 antibody
Formalin-fixed, paraffin-embedded human ovary tissue stained for ATF1 using ab231527 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-ATF1 antibody (ab231527) at 2 µg/ml
Lane 1 : Rat Serum
Lane 2 : Porcine Serum
All lanes : HRP-Linked Guinea Pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 29 kDa
Anti-ATF1 antibody (ab231527) at 2 µg/ml + Recombinant human ATF1 protein.
Predicted band size: 29 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231527 has not yet been referenced specifically in any publications.