Overview

  • Product name

    Anti-ATF1 (phospho S63) antibody
    See all ATF1 primary antibodies
  • Description

    Rabbit polyclonal to ATF1 (phospho S63)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATF1 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ATF1 (phospho Ser63).
    Database link: P18846

  • Positive control

    • WB: HEK-293T and A431 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.

Target

  • Function

    This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
  • Involvement in disease

    Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor 1 antibody
    • ATF 1 antibody
    • atf1 antibody
    • ATF1 EWS fusion gene antibody
    • ATF1 FUS fusion gene antibody
    • ATF1_HUMAN antibody
    • cAMP dependent transcription factor 1 antibody
    • cAMP-dependent transcription factor ATF-1 antibody
    • Cyclic AMP dependent transcription factor ATF 1 antibody
    • Cyclic AMP dependent transcription factor ATF1 antibody
    • Cyclic AMP-dependent transcription factor ATF-1 antibody
    • EWS AFT1 antibody
    • FUS ATF 1 antibody
    • FUS/ATF 1 antibody
    • Protein TREB36 antibody
    • RNA binding protein activating transcription factor 1 fusion protein antibody
    • TREB 36 antibody
    • TREB36 antibody
    • TREB36 protein antibody
    see all

Images

  • All lanes : Anti-ATF1 (phospho S63) antibody (ab226812) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : HEK-293T treated with 25 µg/ml anisomycin for 20 minutes cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa

  • All lanes : Anti-ATF1 (phospho S63) antibody (ab226812) at 1/5000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 2 : A431 treated with 200 ng/ml EGF for 30 minutes cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa

References

ab226812 has not yet been referenced specifically in any publications.

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