Overview

  • Product name

    Anti-ATF1 (phospho S63) antibody [EP1591(2)Y]
    See all ATF1 primary antibodies
  • Description

    Rabbit monoclonal [EP1591(2)Y] to ATF1 (phospho S63)
  • Host species

    Rabbit
  • Specificity

    ab76085 detects ATF1 when phosphorylated at serine 63
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1

  • Positive control

    • WB: HeLa whole cell lysate (ab150035) IHC-P: human breast carcinoma
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1591(2)Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 22052915
WB 1/50000 - 1/100000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
    • Involvement in disease

      Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
    • Sequence similarities

      Belongs to the bZIP family. ATF subfamily.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activating transcription factor 1 antibody
      • ATF 1 antibody
      • atf1 antibody
      • ATF1 EWS fusion gene antibody
      • ATF1 FUS fusion gene antibody
      • ATF1_HUMAN antibody
      • cAMP dependent transcription factor 1 antibody
      • cAMP-dependent transcription factor ATF-1 antibody
      • Cyclic AMP dependent transcription factor ATF 1 antibody
      • Cyclic AMP dependent transcription factor ATF1 antibody
      • Cyclic AMP-dependent transcription factor ATF-1 antibody
      • EWS AFT1 antibody
      • FUS ATF 1 antibody
      • FUS/ATF 1 antibody
      • Protein TREB36 antibody
      • RNA binding protein activating transcription factor 1 fusion protein antibody
      • TREB 36 antibody
      • TREB36 antibody
      • TREB36 protein antibody
      see all

    Images

    • All lanes : Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with Lambda
      Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anit rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

    • Immunohistochemical analysis of ATF1 in paraffin embedded human breast carcinoma tissue, using ab76085 at a 1/100 dilution.

    References

    This product has been referenced in:

    • Li B  et al. Isobavachalcone exerts anti-proliferative and pro-apoptotic effects on human liver cancer cells by targeting the ERKs/RSK2 signaling pathway. Oncol Rep 41:3355-3366 (2019). Read more (PubMed: 30942462) »
    • Upmanyu N  et al. Impairment of the Gna11-controlled expression of claudin-1 and MMP-9 and collective migration of human breast cancer MCF-7 cells by DHEAS. J Steroid Biochem Mol Biol N/A:N/A (2018). Human . Read more (PubMed: 29684479) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your email.

    The concentration of ab76085 batch:GR68333 is 0.2150 mg/ml.

    This a tissue culture supernatant and not affinity purified antibody. As the datasheet indicates ab76085 is a rabbit monoclonal antibody which is generated by a special hybridoma technique so the concentration is different from rabbit polyclonal antibodies. This type of antibodies have higher affinity and specificity. I would advise your customer to consult the datasheet for recommended dilutions for each tested applications.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

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