Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATF2 antibody
See all ATF2 primary antibodies
DescriptionRabbit polyclonal to ATF2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide conjugated to KLH surrounding amino acids 69-73 (VADQTPTPTRFLK) of Human ATF2 (NP_001871.2).
- HeLa cells; HT29 and K562 cell extracts; Human breast carcinoma tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131463 was purified by affinity chromatography using epitope specific peptide.
Our Abpromise guarantee covers the use of ab131463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 65-75 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
All lanes : Anti-ATF2 antibody (ab131463) at 1/500 dilution
Lane 1 : HT29 cell extract
Lane 2 : K562 cell extract
Predicted band size: 55 kDa
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed HeLa cells labelling ATF2 with ab131463 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling ATF2 with ab131463 at 1/50 dilution. The image on the right is preincubated with blocking peptide.
ab131463 has not yet been referenced specifically in any publications.