Key features and details
- Rabbit polyclonal to ATF2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATF2 antibody
See all ATF2 primary antibodies
DescriptionRabbit polyclonal to ATF2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- 293 and 3T3 cell extracts; Human breast carcinoma tissue; HeLa cells.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131484 was purified by affinity chromatography using epitope specific peptide.
Our Abpromise guarantee covers the use of ab131484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 65-75 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionTranscriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Tissue specificityAbundant expression seen in the brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
- Information by UniProt
- Activating transcription factor 2 antibody
- Activating transcription factor 2 splice variant ATF2 var2 antibody
- ATF 2 antibody
All lanes : Anti-ATF2 antibody (ab131484) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 3T3 cell extract
Predicted band size: 55 kDa
Immunofluorescent analysis of methanol-fixed HeLa cells labelling ATF2 with ab131484 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling ATF2 with ab131484 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
ab131484 has been referenced in 1 publication.
- Martins AJ et al. Environment Tunes Propagation of Cell-to-Cell Variation in the Human Macrophage Gene Network. Cell Syst 4:379-392.e12 (2017). PubMed: 28365150